Localization of mRNAs encoding human mitochondrial oxidative phosphorylation proteins

Shinya Matsumoto, Takeshi Uchiumi, Toshiro Saito, Mikako Yagi, Shinya Takazaki, Tomotake Kanki, Dongchon Kang

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The mitochondrial oxidative phosphorylation (OXPHOS) proteins are encoded by both nuclear and mitochondrial DNA. The nuclear-encoded OXPHOS mRNAs have specific subcellular localizations, but little is known about which localize near mitochondria. Here, we compared mRNAs in mitochondria-bound polysome fractions with those in cytosolic, free polysome fractions. mRNAs encoding hydrophobic OXPHOS proteins, which insert into the inner membrane, were localized near mitochondria. Conversely, OXPHOS gene which mRNAs were predominantly localized in cytosol had less than one transmembrane domain. The RNA-binding protein Y-box binding protein-1 is localized at the mitochondrial outer membrane and bound to the OXPHOS mRNAs. Our findings offer new insight into mitochondrial co-translational import in human cells.

Original languageEnglish
Pages (from-to)391-398
Number of pages8
JournalMitochondrion
Volume12
Issue number3
DOIs
Publication statusPublished - May 2012

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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