Localization of NAD-isocitrate dehydrogenase and glutamate dehydrogenase in rice roots: Candidates for providing carbon skeletons to NADH-glutamate synthase

Tomomi Abiko, Mitsuhiro Obara, Akiko Ushioda, Toshihiko Hayakawa, Michael Hodges, Tomoyuki Yamaya

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

In rice roots, transient and cell-type-specific accumulation of both mRNA and protein for NADH-dependent glutamate synthase (NADH-GOGAT) occurs after the supply of NH4+ ions. In order to better understand the origin of 2-oxoglutarate for this reaction, we focused on mitochondrial NAD-dependent isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) in rice roots. Six rice cDNAs encoding a single catalytic (OsIDHa) and two regulatory (OsIDHc;1, OsIDHc;2) IDH subunits and three GDH proteins (OsGDH1-3) were isolated. These genes, except OsGDH3, were expressed in the roots. Real-time PCR analysis showed that OsIDHa and OsIDHc;1 transcripts, but not OsGDH1 and OsGDH2 transcripts, accumulated in a similar manner to NADH-GOGAT mRNA along the crown roots after the supply of different forms of inorganic nitrogen. Furthermore, immunolocalization studies revealed the NH 4+ induction of IDHa protein in two cell layers of the root surface, i.e. epidermis and exodermis, where NADH-GOGAT also accumulated. The possible relationship between NADH-GOGAT, IDH and GDH is discussed. JSPP

Original languageEnglish
Pages (from-to)1724-1734
Number of pages11
JournalPlant and Cell Physiology
Volume46
Issue number10
DOIs
Publication statusPublished - Oct 1 2005

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Glutamate Synthase (NADH)
glutamate synthase (NADH)
Isocitrate Dehydrogenase
Glutamate Dehydrogenase
isocitrate dehydrogenase
glutamate dehydrogenase
Skeleton
skeleton
Carbon
rice
carbon
proteins
epidermis (animal)
quantitative polymerase chain reaction
cells
ions
Messenger RNA
Proteins
nitrogen
Crowns

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Localization of NAD-isocitrate dehydrogenase and glutamate dehydrogenase in rice roots : Candidates for providing carbon skeletons to NADH-glutamate synthase. / Abiko, Tomomi; Obara, Mitsuhiro; Ushioda, Akiko; Hayakawa, Toshihiko; Hodges, Michael; Yamaya, Tomoyuki.

In: Plant and Cell Physiology, Vol. 46, No. 10, 01.10.2005, p. 1724-1734.

Research output: Contribution to journalArticle

Abiko, Tomomi ; Obara, Mitsuhiro ; Ushioda, Akiko ; Hayakawa, Toshihiko ; Hodges, Michael ; Yamaya, Tomoyuki. / Localization of NAD-isocitrate dehydrogenase and glutamate dehydrogenase in rice roots : Candidates for providing carbon skeletons to NADH-glutamate synthase. In: Plant and Cell Physiology. 2005 ; Vol. 46, No. 10. pp. 1724-1734.
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