TY - JOUR
T1 - Localization of neutral ceramidase in caveolin-enriched light membranes of murine endothelial cells
AU - Romiti, Elena
AU - Meacci, Elisabetta
AU - Tanzi, Giuditta
AU - Becciolini, Laura
AU - Mitsutake, Susumu
AU - Farnararo, Marta
AU - Ito, Makoto
AU - Bruni, Paola
N1 - Funding Information:
This work was supported by funds from the Cofinanziamento Ministero dell’ Università e della Ricerca Scientifica e Tecnologica (MURST) Progetti di Interesse Nazionale (PRIN) 1999 and from Ente Cassa di Risparmio di Firenze (to M.F.).
PY - 2001/10/5
Y1 - 2001/10/5
N2 - Sphingomyelinase (SMase) and ceramidase (CDase) activities participate in sphingomyelin (SM) metabolism and have a role in the signal transduction of a variety of ligands. In this study evidence is presented that caveolin-enriched light membranes (CELMs) of murine endothelial cells, characterized by high SM, ceramide (Cer) and cholesterol content, bear acid and neutral SMase as well as neutral CDase activities. Localization of neutral CDase in CELMs was confirmed by Western analysis. Notably, cell treatment with cyclodextrin, which depleted cell cholesterol, did not affect acid or neutral SMase activities but significantly enhanced neutral CDase activity in CELMs, indicating a negative role for cholesterol in CDase regulation. These findings suggest that neutral CDase is implicated, together with SMase activities, in the control of caveolar Cer content that may be critical for caveola dynamics.
AB - Sphingomyelinase (SMase) and ceramidase (CDase) activities participate in sphingomyelin (SM) metabolism and have a role in the signal transduction of a variety of ligands. In this study evidence is presented that caveolin-enriched light membranes (CELMs) of murine endothelial cells, characterized by high SM, ceramide (Cer) and cholesterol content, bear acid and neutral SMase as well as neutral CDase activities. Localization of neutral CDase in CELMs was confirmed by Western analysis. Notably, cell treatment with cyclodextrin, which depleted cell cholesterol, did not affect acid or neutral SMase activities but significantly enhanced neutral CDase activity in CELMs, indicating a negative role for cholesterol in CDase regulation. These findings suggest that neutral CDase is implicated, together with SMase activities, in the control of caveolar Cer content that may be critical for caveola dynamics.
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U2 - 10.1016/S0014-5793(01)02878-2
DO - 10.1016/S0014-5793(01)02878-2
M3 - Article
C2 - 11591392
AN - SCOPUS:0035812885
SN - 0014-5793
VL - 506
SP - 163
EP - 168
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -