Localization of PTP-FERM in nerve processes through its FERM domain

Yohzo Uchida, Masato Ogata, Yoshiko Mori, Masatsugu Oh-hora, Naoya Hatano, Toshiyuki Hamaoka

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    PTP-FERM is a protein tyrosine phosphatase (PTP) of Caenorhabditis elegans containing a FERM domain and a PDZ domain. Here we report the characterization of PTP-FERM and the essential role of its FERM domain in the localization of PTP-FERM in the worm. There are at least three alternatively spliced PTP-FERM isoforms, all of which contain a band 4.1/FERM domain, a PDZ domain, and a catalytic domain. PTP-FERM possessed phosphatase activity. PTP-FERM was expressed predominantly in neurons in the nerve ring and the ventral nerve cord. PTP-FERM was found in the nerve processes and to be enriched in the perimembrane region. Studies using various deletion mutants revealed that the FERM domain was essential and sufficient for the subcellular localization. These results suggest the essential role of the FERM domain in the function of PTP-FERM in the neurons of C. elegans.

    Original languageEnglish
    Pages (from-to)13-19
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume292
    Issue number1
    DOIs
    Publication statusPublished - Jan 1 2002

    Fingerprint

    Protein Tyrosine Phosphatases
    PDZ Domains
    Caenorhabditis elegans
    Neurons
    Phosphoric Monoester Hydrolases
    Catalytic Domain
    Protein Isoforms

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Localization of PTP-FERM in nerve processes through its FERM domain. / Uchida, Yohzo; Ogata, Masato; Mori, Yoshiko; Oh-hora, Masatsugu; Hatano, Naoya; Hamaoka, Toshiyuki.

    In: Biochemical and Biophysical Research Communications, Vol. 292, No. 1, 01.01.2002, p. 13-19.

    Research output: Contribution to journalArticle

    Uchida, Y, Ogata, M, Mori, Y, Oh-hora, M, Hatano, N & Hamaoka, T 2002, 'Localization of PTP-FERM in nerve processes through its FERM domain', Biochemical and Biophysical Research Communications, vol. 292, no. 1, pp. 13-19. https://doi.org/10.1006/bbrc.2002.6613
    Uchida, Yohzo ; Ogata, Masato ; Mori, Yoshiko ; Oh-hora, Masatsugu ; Hatano, Naoya ; Hamaoka, Toshiyuki. / Localization of PTP-FERM in nerve processes through its FERM domain. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 292, No. 1. pp. 13-19.
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