Abstract
The localization of 3H-glucosamine and 35S-sulfate in the hamster retina was studied by light and electron microscopic autoradiography. Exposed silver grains were concentrated in the nerve fiber, inner plexiform, outer plexiform and inner segment layers. The heaviest labeling was observed over the inner segment layer in the glucosamine experiment and over the nerve fiber layers in the sulfate experiment. Specimens from 12- and 16-day-old hamsters showed slightly heavier labeling than did those from adult animals. In the electron microscopic autoradiography using 3H-glucosamine, grains were initially associated with the retinal pigment epithelium, the inner segment and the Muller cell and were subsequently displaced into the spaces between the retinal pigment epithelium and the outer segment and into the nerve fiber layers. The labelled sulfates in the electron microscopic autoradiography were seen in the nerve fiber, plexiform and photoreceptor layers. These data indicate that glycosaminoglycans may be synthesized in the retinal pigment epithelium, the inner segment and the Muller cell.
| Original language | English |
|---|---|
| Pages (from-to) | 119-129 |
| Number of pages | 11 |
| Journal | Japanese Journal of Ophthalmology |
| Volume | 27 |
| Issue number | 1 |
| Publication status | Published - Jan 1 1983 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Ophthalmology
Cite this
Localization of 3H-glucosamine and 35S-sulfate in the hamster retina : Light and electron microscopic autoradiography. / Ohnishi, Y.; Ishibashi, Tatsuro; Taniguchi, Y.
In: Japanese Journal of Ophthalmology, Vol. 27, No. 1, 01.01.1983, p. 119-129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization of 3H-glucosamine and 35S-sulfate in the hamster retina
T2 - Light and electron microscopic autoradiography
AU - Ohnishi, Y.
AU - Ishibashi, Tatsuro
AU - Taniguchi, Y.
PY - 1983/1/1
Y1 - 1983/1/1
N2 - The localization of 3H-glucosamine and 35S-sulfate in the hamster retina was studied by light and electron microscopic autoradiography. Exposed silver grains were concentrated in the nerve fiber, inner plexiform, outer plexiform and inner segment layers. The heaviest labeling was observed over the inner segment layer in the glucosamine experiment and over the nerve fiber layers in the sulfate experiment. Specimens from 12- and 16-day-old hamsters showed slightly heavier labeling than did those from adult animals. In the electron microscopic autoradiography using 3H-glucosamine, grains were initially associated with the retinal pigment epithelium, the inner segment and the Muller cell and were subsequently displaced into the spaces between the retinal pigment epithelium and the outer segment and into the nerve fiber layers. The labelled sulfates in the electron microscopic autoradiography were seen in the nerve fiber, plexiform and photoreceptor layers. These data indicate that glycosaminoglycans may be synthesized in the retinal pigment epithelium, the inner segment and the Muller cell.
AB - The localization of 3H-glucosamine and 35S-sulfate in the hamster retina was studied by light and electron microscopic autoradiography. Exposed silver grains were concentrated in the nerve fiber, inner plexiform, outer plexiform and inner segment layers. The heaviest labeling was observed over the inner segment layer in the glucosamine experiment and over the nerve fiber layers in the sulfate experiment. Specimens from 12- and 16-day-old hamsters showed slightly heavier labeling than did those from adult animals. In the electron microscopic autoradiography using 3H-glucosamine, grains were initially associated with the retinal pigment epithelium, the inner segment and the Muller cell and were subsequently displaced into the spaces between the retinal pigment epithelium and the outer segment and into the nerve fiber layers. The labelled sulfates in the electron microscopic autoradiography were seen in the nerve fiber, plexiform and photoreceptor layers. These data indicate that glycosaminoglycans may be synthesized in the retinal pigment epithelium, the inner segment and the Muller cell.
UR - http://www.scopus.com/inward/record.url?scp=0020644986&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020644986&partnerID=8YFLogxK
M3 - Article
C2 - 6855005
AN - SCOPUS:0020644986
VL - 27
SP - 119
EP - 129
JO - Japanese Journal of Ophthalmology
JF - Japanese Journal of Ophthalmology
SN - 0021-5155
IS - 1
ER -