Localization of Tektin 1 at both acrosome and Flagella of mouse and bull spermatozoa

Sayoko Oiki, Erina Hiyama, Takafumi Gotoh, Hiroshi Iida

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Tektins (TEKTs) are constitutive filamentous proteins of microtubules in cilia, flagella, basal bodies, and centrioles. In mammals, five TEKTs (TEKT1, 2, 3, 4, and 5) have been identified in testis and spermatozoa. With the exception of TEKT1, these TEKTs have been reported to be present in spermatozoa with predominant localization at the peri-axoneme structures of flagella, i.e., mitochondria and outer dense fibers. In the present study, we produced an antibody against TEKT1 to examine the localization of TEKT1 in mouse, bull, and rat spermatozoa. By immunoblot analyses and immunofluorescence microscopy, we found TEKT1 to be present in sperm flagella and at the apical region of acrosome cap in spermatozoa of all these species. Acrosome-associated TEKT1 disappeared after in vitro acrosome reaction in mouse spermatozoa. These observations suggest another potential role for TEKT1 as a cytoskeletal element in the sperm head, or as a molecule involved in acrosome-related phenomena, such as acrosome reaction.

Original languageEnglish
Pages (from-to)101-107
Number of pages7
JournalZoological science
Volume31
Issue number2
DOIs
Publication statusPublished - Feb 1 2014

Fingerprint

acrosome
flagellum
bulls
spermatozoa
mice
acrosome reaction
centrioles
cilia
fluorescence microscopy
microtubules
testes
mitochondria
mammals
antibodies
rats
proteins

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Localization of Tektin 1 at both acrosome and Flagella of mouse and bull spermatozoa. / Oiki, Sayoko; Hiyama, Erina; Gotoh, Takafumi; Iida, Hiroshi.

In: Zoological science, Vol. 31, No. 2, 01.02.2014, p. 101-107.

Research output: Contribution to journalArticle

Oiki, Sayoko ; Hiyama, Erina ; Gotoh, Takafumi ; Iida, Hiroshi. / Localization of Tektin 1 at both acrosome and Flagella of mouse and bull spermatozoa. In: Zoological science. 2014 ; Vol. 31, No. 2. pp. 101-107.
@article{0a35da82a2ee4d9cb646ecceeae01173,
title = "Localization of Tektin 1 at both acrosome and Flagella of mouse and bull spermatozoa",
abstract = "Tektins (TEKTs) are constitutive filamentous proteins of microtubules in cilia, flagella, basal bodies, and centrioles. In mammals, five TEKTs (TEKT1, 2, 3, 4, and 5) have been identified in testis and spermatozoa. With the exception of TEKT1, these TEKTs have been reported to be present in spermatozoa with predominant localization at the peri-axoneme structures of flagella, i.e., mitochondria and outer dense fibers. In the present study, we produced an antibody against TEKT1 to examine the localization of TEKT1 in mouse, bull, and rat spermatozoa. By immunoblot analyses and immunofluorescence microscopy, we found TEKT1 to be present in sperm flagella and at the apical region of acrosome cap in spermatozoa of all these species. Acrosome-associated TEKT1 disappeared after in vitro acrosome reaction in mouse spermatozoa. These observations suggest another potential role for TEKT1 as a cytoskeletal element in the sperm head, or as a molecule involved in acrosome-related phenomena, such as acrosome reaction.",
author = "Sayoko Oiki and Erina Hiyama and Takafumi Gotoh and Hiroshi Iida",
year = "2014",
month = "2",
day = "1",
doi = "10.2108/zsj.31.101",
language = "English",
volume = "31",
pages = "101--107",
journal = "Zoological Science",
issn = "0289-0003",
publisher = "Zoological Society of Japan",
number = "2",

}

TY - JOUR

T1 - Localization of Tektin 1 at both acrosome and Flagella of mouse and bull spermatozoa

AU - Oiki, Sayoko

AU - Hiyama, Erina

AU - Gotoh, Takafumi

AU - Iida, Hiroshi

PY - 2014/2/1

Y1 - 2014/2/1

N2 - Tektins (TEKTs) are constitutive filamentous proteins of microtubules in cilia, flagella, basal bodies, and centrioles. In mammals, five TEKTs (TEKT1, 2, 3, 4, and 5) have been identified in testis and spermatozoa. With the exception of TEKT1, these TEKTs have been reported to be present in spermatozoa with predominant localization at the peri-axoneme structures of flagella, i.e., mitochondria and outer dense fibers. In the present study, we produced an antibody against TEKT1 to examine the localization of TEKT1 in mouse, bull, and rat spermatozoa. By immunoblot analyses and immunofluorescence microscopy, we found TEKT1 to be present in sperm flagella and at the apical region of acrosome cap in spermatozoa of all these species. Acrosome-associated TEKT1 disappeared after in vitro acrosome reaction in mouse spermatozoa. These observations suggest another potential role for TEKT1 as a cytoskeletal element in the sperm head, or as a molecule involved in acrosome-related phenomena, such as acrosome reaction.

AB - Tektins (TEKTs) are constitutive filamentous proteins of microtubules in cilia, flagella, basal bodies, and centrioles. In mammals, five TEKTs (TEKT1, 2, 3, 4, and 5) have been identified in testis and spermatozoa. With the exception of TEKT1, these TEKTs have been reported to be present in spermatozoa with predominant localization at the peri-axoneme structures of flagella, i.e., mitochondria and outer dense fibers. In the present study, we produced an antibody against TEKT1 to examine the localization of TEKT1 in mouse, bull, and rat spermatozoa. By immunoblot analyses and immunofluorescence microscopy, we found TEKT1 to be present in sperm flagella and at the apical region of acrosome cap in spermatozoa of all these species. Acrosome-associated TEKT1 disappeared after in vitro acrosome reaction in mouse spermatozoa. These observations suggest another potential role for TEKT1 as a cytoskeletal element in the sperm head, or as a molecule involved in acrosome-related phenomena, such as acrosome reaction.

UR - http://www.scopus.com/inward/record.url?scp=84894095539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894095539&partnerID=8YFLogxK

U2 - 10.2108/zsj.31.101

DO - 10.2108/zsj.31.101

M3 - Article

C2 - 24521320

AN - SCOPUS:84894095539

VL - 31

SP - 101

EP - 107

JO - Zoological Science

JF - Zoological Science

SN - 0289-0003

IS - 2

ER -