Localization of vascular adhesion protein-1 (VAP-1) in the human eye

Lama Almulki, Kousuke Noda, Shintaro Nakao, Toshio Hisatomi, Kennard L. Thomas, Ali Hafezi-Moghadam

Research output: Contribution to journalArticle

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Abstract

Recently we showed a critical role for Vascular Adhesion Protein-1 (VAP-1) in rodents during acute ocular inflammation, angiogenesis, and diabetic retinal leukostasis. However, the expression of VAP-1 in the human eye is unknown. VAP-1 localization was therefore investigated by immunohistochemistry. Five micrometer thick sections were generated from human ocular tissues embedded in paraffin. Sections were incubated overnight with primary mAbs against VAP-1 (5 μg/ml), smooth muscle actin (1 μg/ml), CD31 or isotype-matched IgG at 4 °C. Subsequently, a secondary mAb was used for 30 min at room temperature, followed by Dako Envision + HRP (AEC) System for signal detection. The stained sections were examined using light microscopy and the signal intensity was quantified by two evaluators and graded into 4 discrete categories. In all examined ocular tissues, VAP-1 staining was confined to the vasculature. VAP-1 labeling showed the highest intensity in both arteries and veins of neuronal tissues: retina and optic nerve, and the lowest intensity in the iris vasculature (p < 0.05). Scleral and choroidal vessels showed moderate staining for VAP-1. VAP-1 intensity was significantly higher in the arteries compared to veins (p < 0.05). Furthermore, VAP-1 staining in arteries colocalized with both CD31 and smooth muscle actin (sm-actin) staining, suggesting expression of VAP-1 in endothelial cells, smooth muscle cells or potentially pericytes. In conclusion, immunohistochemistry reveals constitutive expression of VAP-1 in human ocular tissues. VAP-1 expression is nearly exclusive to the vasculature with arteries showing significantly higher expression than veins. Furthermore, VAP-1 expression in the ocular vasculature is heterogeneous, with the vessels of the optic nerve and the retina showing highest expressions. These results characterize VAP-1 expression in human ocular tissues.

Original languageEnglish
Pages (from-to)26-32
Number of pages7
JournalExperimental Eye Research
Volume90
Issue number1
DOIs
Publication statusPublished - Jan 1 2010

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Blood Vessels
Proteins
Arteries
Staining and Labeling
Veins
Optic Nerve
Smooth Muscle
Retina
human AOC3 protein
Actins
Leukostasis
Immunohistochemistry
Tissue Adhesions
Pericytes
Iris
Paraffin
Smooth Muscle Myocytes
Microscopy
Rodentia
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Almulki, L., Noda, K., Nakao, S., Hisatomi, T., Thomas, K. L., & Hafezi-Moghadam, A. (2010). Localization of vascular adhesion protein-1 (VAP-1) in the human eye. Experimental Eye Research, 90(1), 26-32. https://doi.org/10.1016/j.exer.2009.09.005

Localization of vascular adhesion protein-1 (VAP-1) in the human eye. / Almulki, Lama; Noda, Kousuke; Nakao, Shintaro; Hisatomi, Toshio; Thomas, Kennard L.; Hafezi-Moghadam, Ali.

In: Experimental Eye Research, Vol. 90, No. 1, 01.01.2010, p. 26-32.

Research output: Contribution to journalArticle

Almulki, L, Noda, K, Nakao, S, Hisatomi, T, Thomas, KL & Hafezi-Moghadam, A 2010, 'Localization of vascular adhesion protein-1 (VAP-1) in the human eye', Experimental Eye Research, vol. 90, no. 1, pp. 26-32. https://doi.org/10.1016/j.exer.2009.09.005
Almulki, Lama ; Noda, Kousuke ; Nakao, Shintaro ; Hisatomi, Toshio ; Thomas, Kennard L. ; Hafezi-Moghadam, Ali. / Localization of vascular adhesion protein-1 (VAP-1) in the human eye. In: Experimental Eye Research. 2010 ; Vol. 90, No. 1. pp. 26-32.
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