Location of the binding region for 23S ribosomal RNA on ribosomal protein L2 from Bacillus stearothermophilus

Keiichi WATANABE, Makoto KIMURA

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Tryptic and chymotryptic digestions of a complex between ribosomal protein L2 and the 23S RNA of Bacillus stearothermophilus produced two protected fragments (designated TF and CF). These protein fragments remained tightly bound to the RNA and were able to rebind specifically to 23S RNA. Amino acid sequence analysis of the isolated fragments revealed that both fragments TF and CF originated from the central half of the protein L2 (positions 60–206 and 58–201) with molecular masses of 15918 Da and 15690 Da respectively. From these results it is concluded that the protected central half is the primary RNA‐binding region of the protein L2. A combination of these and other results suggests that the N and/or C‐terminal (but not the central) regions may be involved in the peptidyltransferase activity of the 50S subunit.

Original languageEnglish
Pages (from-to)299-304
Number of pages6
JournalEuropean Journal of Biochemistry
Volume153
Issue number2
DOIs
Publication statusPublished - Dec 1985

All Science Journal Classification (ASJC) codes

  • Biochemistry

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