Log P effect of organic solvents on a thermophilic alcohol dehydrogenase

Hidehiko Hirakawa, Noriho Kamiya, Yutaka Kawarabayashi, Teruyuki Nagamune

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

An alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix was activated by water-miscible organic solvents. This activation was influenced by the kind and the concentration of the added organic solvents. The kcat was increased by a factor of over ten when the mole fraction of acetonitrile was 0.1. This effect was large when organic solvents with large log P values were added. In fact, the kcat showed a strong positive correlation with the log P value of the mixed solvent at a constant mole fraction of water, while it was not affected by the kind of organic solvents added. Both the activation enthalpy and the entropy decreased with an increase in log P. The contribution of the activation enthalpy to the free energy of activation was larger than that of the activation entropy, and the free energy of activation decreased with an increase in log P.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1748
Issue number1
DOIs
Publication statusPublished - Apr 15 2005

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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