Long circulation of intravenously administered plasmid DNA delivered with dendritic poly(l-lysine) in the blood flow

Takahito Kawano, Tatsuya Okuda, Haruhiko Aoyagi, Takuro Niidome

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

We previously showed that dendritic poly(l-lysine) of the 6th generation (KG6) had high transfection ability without significant cytotoxicity in vitro. Here, to evaluate the potential of KG6 as a nonviral gene carrier that works in vivo, we investigated the biodistribution of plasmid DNA delivered with KG6 in mice after intravenous administration, in comparison with DOTAP/Chol liposomes and PEI. Southern blotting analysis revealed that plasmid DNA complexes with KG6 at a C/A ratio of 8.0 circulated in the blood for 3 h after intravenous injection. The amounts of plasmid DNA in the liver gradually decreased. In tumor-bearing mice, plasmid DNA injected with KG6 was observed in the tumor at 60 min after the intravenous injection, while no DNA was present in the tumor using DOTAP/Chol liposomes. The stealth character of DNA complexes with KG6 in the blood would cause an enhanced permeability and retention (EPR) effect in the tumor. KG6 is expected to be a promising candidate that enables functional gene delivery in vivo.

Original languageEnglish
Pages (from-to)329-337
Number of pages9
JournalJournal of Controlled Release
Volume99
Issue number2
DOIs
Publication statusPublished - Sep 30 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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