LONG GRAIN 1: a novel gene that regulates grain length in rice

Ryouhei Morita, Hiroyuki Ichida, Kotaro Ishii, Yoriko Hayashi, Hiroshi Abe, Yuki Shirakawa, Katsunori Ichinose, Kazuhide Tsuneizumi, Tomohiko Kazama, Kinya Toriyama, Tadashi Sato, Tomoko Abe

Research output: Contribution to journalArticle

Abstract

Grain size is one of the most important traits that influence grain yield in rice. Here we report the isolation and characterization of the long grain1 (lin1) mutant, which exhibited long grains and increased grain weight. A field trial conducted over 2 years indicated that the grain weight increased 3.0–6.6% in the lin1 mutant compared with that of the wild-type Nipponbare. Whole-genome sequencing and genetic linkage analysis indicated that a 1-bp deletion within the coding sequence of Os06g0675200, which showed no homology to previously characterized genes, is responsible for the lin1 phenotype. Quantitative real-time PCR indicated that LIN1 transcripts were more abundant in young panicles than at advanced inflorescence developmental stages, which indicated that LIN1 controls grain length mainly at early stages of grain development. Sequence polymorphism analysis of LIN1 showed that all 15 temperate japonica cultivars tested as well as six out of the nine indica cultivars tested possessed the Nipponbare-type LIN1 allele, whereas the remaining three indica cultivars and one aus cultivar tested harbored an identical missense mutation in LIN1. These results revealed that the mutant allele of LIN1 has not been widely utilized in breeding temperate japonica cultivars currently in cultivation. Our findings indicate that the lin1 mutation may be useful to further improve grain length and presumably grain yield in temperate japonica and indica rice cultivars that harbor the Nipponbare-type LIN1 allele.

Original languageEnglish
Article number135
JournalMolecular Breeding
Volume39
Issue number9
DOIs
Publication statusPublished - Sep 1 2019
Externally publishedYes

Fingerprint

Alleles
rice
cultivars
Genes
Weights and Measures
Inflorescence
Genetic Linkage
genes
Missense Mutation
alleles
mutants
Breeding
Sequence Analysis
Real-Time Polymerase Chain Reaction
inflorescences
grain yield
Genome
Phenotype
Mutation
missense mutation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Morita, R., Ichida, H., Ishii, K., Hayashi, Y., Abe, H., Shirakawa, Y., ... Abe, T. (2019). LONG GRAIN 1: a novel gene that regulates grain length in rice. Molecular Breeding, 39(9), [135]. https://doi.org/10.1007/s11032-019-1032-1

LONG GRAIN 1 : a novel gene that regulates grain length in rice. / Morita, Ryouhei; Ichida, Hiroyuki; Ishii, Kotaro; Hayashi, Yoriko; Abe, Hiroshi; Shirakawa, Yuki; Ichinose, Katsunori; Tsuneizumi, Kazuhide; Kazama, Tomohiko; Toriyama, Kinya; Sato, Tadashi; Abe, Tomoko.

In: Molecular Breeding, Vol. 39, No. 9, 135, 01.09.2019.

Research output: Contribution to journalArticle

Morita, R, Ichida, H, Ishii, K, Hayashi, Y, Abe, H, Shirakawa, Y, Ichinose, K, Tsuneizumi, K, Kazama, T, Toriyama, K, Sato, T & Abe, T 2019, 'LONG GRAIN 1: a novel gene that regulates grain length in rice', Molecular Breeding, vol. 39, no. 9, 135. https://doi.org/10.1007/s11032-019-1032-1
Morita R, Ichida H, Ishii K, Hayashi Y, Abe H, Shirakawa Y et al. LONG GRAIN 1: a novel gene that regulates grain length in rice. Molecular Breeding. 2019 Sep 1;39(9). 135. https://doi.org/10.1007/s11032-019-1032-1
Morita, Ryouhei ; Ichida, Hiroyuki ; Ishii, Kotaro ; Hayashi, Yoriko ; Abe, Hiroshi ; Shirakawa, Yuki ; Ichinose, Katsunori ; Tsuneizumi, Kazuhide ; Kazama, Tomohiko ; Toriyama, Kinya ; Sato, Tadashi ; Abe, Tomoko. / LONG GRAIN 1 : a novel gene that regulates grain length in rice. In: Molecular Breeding. 2019 ; Vol. 39, No. 9.
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