Long-term continuous production of optically active 2-(4-chlorophenoxy)propanoic acid by yeast lipase in an organic solvent system

Toshiaki Fukui, Takuo Kawamoto, Kenji Sonomoto, Atsuo Tanaka

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Long-term continuous optical resolution of 2-(4-chlorophenoxy)propanoic acid was carried out by stereoselective esterification with Celite-adsorbed lipase OF 360 from Candida cylindracea using n-tetradecanol as the second substrate in organic solvent systems. The water content of the Celite-adsorbed lipase affected productivity, 1.0 μl water·mg lipase-1 being optimal for preparation of the adsorbed lipase. Water-saturated carbon tetrachloride-isooctane (8:2, v/v) was found to be an excellent organic solvent for the continuous operation. The particle size of Celite had no effect on productivity. Under optimized conditions, the (R)-enantiomer of the acid was continuously esterified with high stereoselectivity in a packed-bed column reactor for 34 days. Furthermore, it was found that treatment of the reactor with acetone made it possible to restore productivity and extend the period of continuous operation for further 29 days.

Original languageEnglish
Pages (from-to)330-334
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume34
Issue number3
DOIs
Publication statusPublished - Dec 1 1990

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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