Long-Term Exercise Protects against Cellular Stresses in Aged Mice

Irina Belaya, Masataka Suwa, Tao Chen, Rashid Giniatullin, Katja M. Kanninen, Mustafa Atalay, Shuzo Kumagai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The current study examined the effect of aging and long-term wheel-running on the expression of heat shock protein (HSP), redox regulation, and endoplasmic reticulum (ER) stress markers in tibialis anterior (T.A.) and soleus muscle of mice. Male mice were divided into young (Y, 3-month-old), old-sedentary (OS, 24-month-old), and old-exercise (OE, 24-month-old) groups. The OE group started voluntary wheel-running at 3 months and continued until 24 months of age. Aging was associated with a higher thioredoxin-interacting protein (TxNiP) level, lower thioredoxin-1 (TRX-1) to TxNiP ratio-a determinant of redox regulation and increased CHOP, an indicator of ER stress-related apoptosis signaling in both muscles. Notably, GRP78, a key indicator of ER stress, was selectively elevated in T.A. Long-term exercise decreased TxNiP in T.A. and soleus muscles and increased the TRX-1/TxNiP ratio in soleus muscle of aged mice. Inducible HSP70 and constituent HSC70 were upregulated, whereas CHOP was reduced after exercise in soleus muscle. Thus, our data demonstrated that aging induced oxidative stress and activated ER stress-related apoptosis signaling in skeletal muscle, whereas long-term wheel-running improved redox regulation, ER stress adaptation and attenuated ER stress-related apoptosis signaling. These findings suggest that life-long exercise can protect against age-related cellular stress.

Original languageEnglish
Number of pages1
JournalOxidative medicine and cellular longevity
Volume2018
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Thioredoxins
Endoplasmic Reticulum Stress
Muscle
Skeletal Muscle
Running
Oxidation-Reduction
Wheels
Aging of materials
Apoptosis
Proteins
Oxidative stress
Heat-Shock Proteins
Oxidative Stress
Muscles

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ageing
  • Cell Biology

Cite this

Long-Term Exercise Protects against Cellular Stresses in Aged Mice. / Belaya, Irina; Suwa, Masataka; Chen, Tao; Giniatullin, Rashid; Kanninen, Katja M.; Atalay, Mustafa; Kumagai, Shuzo.

In: Oxidative medicine and cellular longevity, Vol. 2018, 01.01.2018.

Research output: Contribution to journalArticle

Belaya, Irina ; Suwa, Masataka ; Chen, Tao ; Giniatullin, Rashid ; Kanninen, Katja M. ; Atalay, Mustafa ; Kumagai, Shuzo. / Long-Term Exercise Protects against Cellular Stresses in Aged Mice. In: Oxidative medicine and cellular longevity. 2018 ; Vol. 2018.
@article{7df107b2bc8346d598571592d0d3ce0a,
title = "Long-Term Exercise Protects against Cellular Stresses in Aged Mice",
abstract = "The current study examined the effect of aging and long-term wheel-running on the expression of heat shock protein (HSP), redox regulation, and endoplasmic reticulum (ER) stress markers in tibialis anterior (T.A.) and soleus muscle of mice. Male mice were divided into young (Y, 3-month-old), old-sedentary (OS, 24-month-old), and old-exercise (OE, 24-month-old) groups. The OE group started voluntary wheel-running at 3 months and continued until 24 months of age. Aging was associated with a higher thioredoxin-interacting protein (TxNiP) level, lower thioredoxin-1 (TRX-1) to TxNiP ratio-a determinant of redox regulation and increased CHOP, an indicator of ER stress-related apoptosis signaling in both muscles. Notably, GRP78, a key indicator of ER stress, was selectively elevated in T.A. Long-term exercise decreased TxNiP in T.A. and soleus muscles and increased the TRX-1/TxNiP ratio in soleus muscle of aged mice. Inducible HSP70 and constituent HSC70 were upregulated, whereas CHOP was reduced after exercise in soleus muscle. Thus, our data demonstrated that aging induced oxidative stress and activated ER stress-related apoptosis signaling in skeletal muscle, whereas long-term wheel-running improved redox regulation, ER stress adaptation and attenuated ER stress-related apoptosis signaling. These findings suggest that life-long exercise can protect against age-related cellular stress.",
author = "Irina Belaya and Masataka Suwa and Tao Chen and Rashid Giniatullin and Kanninen, {Katja M.} and Mustafa Atalay and Shuzo Kumagai",
year = "2018",
month = "1",
day = "1",
doi = "10.1155/2018/2894247",
language = "English",
volume = "2018",
journal = "Oxidative Medicine and Cellular Longevity",
issn = "1942-0900",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Long-Term Exercise Protects against Cellular Stresses in Aged Mice

AU - Belaya, Irina

AU - Suwa, Masataka

AU - Chen, Tao

AU - Giniatullin, Rashid

AU - Kanninen, Katja M.

AU - Atalay, Mustafa

AU - Kumagai, Shuzo

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The current study examined the effect of aging and long-term wheel-running on the expression of heat shock protein (HSP), redox regulation, and endoplasmic reticulum (ER) stress markers in tibialis anterior (T.A.) and soleus muscle of mice. Male mice were divided into young (Y, 3-month-old), old-sedentary (OS, 24-month-old), and old-exercise (OE, 24-month-old) groups. The OE group started voluntary wheel-running at 3 months and continued until 24 months of age. Aging was associated with a higher thioredoxin-interacting protein (TxNiP) level, lower thioredoxin-1 (TRX-1) to TxNiP ratio-a determinant of redox regulation and increased CHOP, an indicator of ER stress-related apoptosis signaling in both muscles. Notably, GRP78, a key indicator of ER stress, was selectively elevated in T.A. Long-term exercise decreased TxNiP in T.A. and soleus muscles and increased the TRX-1/TxNiP ratio in soleus muscle of aged mice. Inducible HSP70 and constituent HSC70 were upregulated, whereas CHOP was reduced after exercise in soleus muscle. Thus, our data demonstrated that aging induced oxidative stress and activated ER stress-related apoptosis signaling in skeletal muscle, whereas long-term wheel-running improved redox regulation, ER stress adaptation and attenuated ER stress-related apoptosis signaling. These findings suggest that life-long exercise can protect against age-related cellular stress.

AB - The current study examined the effect of aging and long-term wheel-running on the expression of heat shock protein (HSP), redox regulation, and endoplasmic reticulum (ER) stress markers in tibialis anterior (T.A.) and soleus muscle of mice. Male mice were divided into young (Y, 3-month-old), old-sedentary (OS, 24-month-old), and old-exercise (OE, 24-month-old) groups. The OE group started voluntary wheel-running at 3 months and continued until 24 months of age. Aging was associated with a higher thioredoxin-interacting protein (TxNiP) level, lower thioredoxin-1 (TRX-1) to TxNiP ratio-a determinant of redox regulation and increased CHOP, an indicator of ER stress-related apoptosis signaling in both muscles. Notably, GRP78, a key indicator of ER stress, was selectively elevated in T.A. Long-term exercise decreased TxNiP in T.A. and soleus muscles and increased the TRX-1/TxNiP ratio in soleus muscle of aged mice. Inducible HSP70 and constituent HSC70 were upregulated, whereas CHOP was reduced after exercise in soleus muscle. Thus, our data demonstrated that aging induced oxidative stress and activated ER stress-related apoptosis signaling in skeletal muscle, whereas long-term wheel-running improved redox regulation, ER stress adaptation and attenuated ER stress-related apoptosis signaling. These findings suggest that life-long exercise can protect against age-related cellular stress.

UR - http://www.scopus.com/inward/record.url?scp=85054459935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054459935&partnerID=8YFLogxK

U2 - 10.1155/2018/2894247

DO - 10.1155/2018/2894247

M3 - Article

VL - 2018

JO - Oxidative Medicine and Cellular Longevity

JF - Oxidative Medicine and Cellular Longevity

SN - 1942-0900

ER -