Long-term spatial cognitive impairment after middle cerebral artery occlusion in rats: No involvement of the hippocampus

Mitsuko Okada, Hiroshi Nakanishi, Akira Tamura, Akinori Urae, Kazunori Mine, Kenji Yamamoto, Michihiro Fujiwara

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The behavioral and neurochemical changes in the chronic phase of permanent occlusion of the right middle cerebral artery (MCA) in rats were investigated. One month after MCA occlusion, 23 rats were unable to solve a radial eight-arm maze task during an entire 1-month period, whereas seven rats were able to solve this task. Three months after occlusion, 19 MCA- occluded rats failed to solve the task successfully again for at least 1 month (the cognitively impaired rats), whereas 11 MCA-occluded rats were able to solve it (the cognitively unimpaired rats). The rats that underwent behavioral testing were examined for any changes in the acetylcholine (ACh) levels in the hippocampus using HPLC with electrochemical detection or the formation of long-term potentiation (LTP) in the population spike of the hippocampal CA1 field. The immunohistochemical distribution of either the microtubule-associated protein 2 (MAP2) or glial fibrillary acidic protein (GFAP) in the hippocampus of the cognitively impaired rats was also studied. In the cognitively impaired rats, neither the suppression of the induction of LTP, nor the degradation of MAP2, nor the increase in the GFAP immunoreactivity was observed in the hippocampus. The levels of ACh in the hippocampus did not change significantly among the cognitively impaired, unimpaired, and the sham-operated rats. These results suggest that MCA occlusion is capable of producing long-term spatial cognitive disturbance in rats without any evidence of neurobiological damage in the hippocampus.

Original languageEnglish
Pages (from-to)1012-1021
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume15
Issue number6
DOIs
Publication statusPublished - Jan 1 1995

Fingerprint

Middle Cerebral Artery Infarction
Hippocampus
Microtubule-Associated Proteins
Long-Term Potentiation
Glial Fibrillary Acidic Protein
Acetylcholine
Cognitive Dysfunction
Middle Cerebral Artery
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Long-term spatial cognitive impairment after middle cerebral artery occlusion in rats : No involvement of the hippocampus. / Okada, Mitsuko; Nakanishi, Hiroshi; Tamura, Akira; Urae, Akinori; Mine, Kazunori; Yamamoto, Kenji; Fujiwara, Michihiro.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 15, No. 6, 01.01.1995, p. 1012-1021.

Research output: Contribution to journalArticle

Okada, Mitsuko ; Nakanishi, Hiroshi ; Tamura, Akira ; Urae, Akinori ; Mine, Kazunori ; Yamamoto, Kenji ; Fujiwara, Michihiro. / Long-term spatial cognitive impairment after middle cerebral artery occlusion in rats : No involvement of the hippocampus. In: Journal of Cerebral Blood Flow and Metabolism. 1995 ; Vol. 15, No. 6. pp. 1012-1021.
@article{639a70859ed0482fbef8399254c6bb66,
title = "Long-term spatial cognitive impairment after middle cerebral artery occlusion in rats: No involvement of the hippocampus",
abstract = "The behavioral and neurochemical changes in the chronic phase of permanent occlusion of the right middle cerebral artery (MCA) in rats were investigated. One month after MCA occlusion, 23 rats were unable to solve a radial eight-arm maze task during an entire 1-month period, whereas seven rats were able to solve this task. Three months after occlusion, 19 MCA- occluded rats failed to solve the task successfully again for at least 1 month (the cognitively impaired rats), whereas 11 MCA-occluded rats were able to solve it (the cognitively unimpaired rats). The rats that underwent behavioral testing were examined for any changes in the acetylcholine (ACh) levels in the hippocampus using HPLC with electrochemical detection or the formation of long-term potentiation (LTP) in the population spike of the hippocampal CA1 field. The immunohistochemical distribution of either the microtubule-associated protein 2 (MAP2) or glial fibrillary acidic protein (GFAP) in the hippocampus of the cognitively impaired rats was also studied. In the cognitively impaired rats, neither the suppression of the induction of LTP, nor the degradation of MAP2, nor the increase in the GFAP immunoreactivity was observed in the hippocampus. The levels of ACh in the hippocampus did not change significantly among the cognitively impaired, unimpaired, and the sham-operated rats. These results suggest that MCA occlusion is capable of producing long-term spatial cognitive disturbance in rats without any evidence of neurobiological damage in the hippocampus.",
author = "Mitsuko Okada and Hiroshi Nakanishi and Akira Tamura and Akinori Urae and Kazunori Mine and Kenji Yamamoto and Michihiro Fujiwara",
year = "1995",
month = "1",
day = "1",
doi = "10.1038/jcbfm.1995.127",
language = "English",
volume = "15",
pages = "1012--1021",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Long-term spatial cognitive impairment after middle cerebral artery occlusion in rats

T2 - No involvement of the hippocampus

AU - Okada, Mitsuko

AU - Nakanishi, Hiroshi

AU - Tamura, Akira

AU - Urae, Akinori

AU - Mine, Kazunori

AU - Yamamoto, Kenji

AU - Fujiwara, Michihiro

PY - 1995/1/1

Y1 - 1995/1/1

N2 - The behavioral and neurochemical changes in the chronic phase of permanent occlusion of the right middle cerebral artery (MCA) in rats were investigated. One month after MCA occlusion, 23 rats were unable to solve a radial eight-arm maze task during an entire 1-month period, whereas seven rats were able to solve this task. Three months after occlusion, 19 MCA- occluded rats failed to solve the task successfully again for at least 1 month (the cognitively impaired rats), whereas 11 MCA-occluded rats were able to solve it (the cognitively unimpaired rats). The rats that underwent behavioral testing were examined for any changes in the acetylcholine (ACh) levels in the hippocampus using HPLC with electrochemical detection or the formation of long-term potentiation (LTP) in the population spike of the hippocampal CA1 field. The immunohistochemical distribution of either the microtubule-associated protein 2 (MAP2) or glial fibrillary acidic protein (GFAP) in the hippocampus of the cognitively impaired rats was also studied. In the cognitively impaired rats, neither the suppression of the induction of LTP, nor the degradation of MAP2, nor the increase in the GFAP immunoreactivity was observed in the hippocampus. The levels of ACh in the hippocampus did not change significantly among the cognitively impaired, unimpaired, and the sham-operated rats. These results suggest that MCA occlusion is capable of producing long-term spatial cognitive disturbance in rats without any evidence of neurobiological damage in the hippocampus.

AB - The behavioral and neurochemical changes in the chronic phase of permanent occlusion of the right middle cerebral artery (MCA) in rats were investigated. One month after MCA occlusion, 23 rats were unable to solve a radial eight-arm maze task during an entire 1-month period, whereas seven rats were able to solve this task. Three months after occlusion, 19 MCA- occluded rats failed to solve the task successfully again for at least 1 month (the cognitively impaired rats), whereas 11 MCA-occluded rats were able to solve it (the cognitively unimpaired rats). The rats that underwent behavioral testing were examined for any changes in the acetylcholine (ACh) levels in the hippocampus using HPLC with electrochemical detection or the formation of long-term potentiation (LTP) in the population spike of the hippocampal CA1 field. The immunohistochemical distribution of either the microtubule-associated protein 2 (MAP2) or glial fibrillary acidic protein (GFAP) in the hippocampus of the cognitively impaired rats was also studied. In the cognitively impaired rats, neither the suppression of the induction of LTP, nor the degradation of MAP2, nor the increase in the GFAP immunoreactivity was observed in the hippocampus. The levels of ACh in the hippocampus did not change significantly among the cognitively impaired, unimpaired, and the sham-operated rats. These results suggest that MCA occlusion is capable of producing long-term spatial cognitive disturbance in rats without any evidence of neurobiological damage in the hippocampus.

UR - http://www.scopus.com/inward/record.url?scp=0028858602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028858602&partnerID=8YFLogxK

U2 - 10.1038/jcbfm.1995.127

DO - 10.1038/jcbfm.1995.127

M3 - Article

C2 - 7593333

AN - SCOPUS:0028858602

VL - 15

SP - 1012

EP - 1021

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 6

ER -