Loss of a GPI-anchored membrane protein Aah3p causes a defect in vacuolar protein sorting in Schizosaccharomyces pombe

Tomoko Iwaki, Tomotake Morita, Naotaka Tanaka, Yuko Giga-Hama, Kaoru Takegawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Schizosaccharomyces pombe has four α-amylase homologs (Aah1p-Aah4p) with a glycosylphosphatidylinositol (GPI) modification site at the C-terminal end. Disruption mutants of aah genes were tested for mislocalization of vacuolar carboxypeptidase Y (CPY), and aah3Δ was found to secrete CPY. The conversion rate from pro- to mature CPY was greatly impaired in aah3Δ, and fluorescence microscopy inidicated that a sorting receptor for CPY, Vps10p, mislocalized to the vacuolar membrane. These results indicate that aah3Δ had a defect in the retrograde transport of Vps10p, and that Aah3p is the first S. pombe specific protein required for vacuolar protein sorting.

Original languageEnglish
Pages (from-to)623-626
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number2
DOIs
Publication statusPublished - Mar 1 2007
Externally publishedYes

Fingerprint

Cathepsin A
Glycosylphosphatidylinositols
Schizosaccharomyces
Protein Transport
Sorting
Membrane Proteins
Defects
Proteins
Schizosaccharomyces pombe Proteins
Fluorescence microscopy
Amylases
Fluorescence Microscopy
Genes
Membranes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Loss of a GPI-anchored membrane protein Aah3p causes a defect in vacuolar protein sorting in Schizosaccharomyces pombe. / Iwaki, Tomoko; Morita, Tomotake; Tanaka, Naotaka; Giga-Hama, Yuko; Takegawa, Kaoru.

In: Bioscience, Biotechnology and Biochemistry, Vol. 71, No. 2, 01.03.2007, p. 623-626.

Research output: Contribution to journalArticle

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