Loss of constitutional heterozygosity in colon carcinoma from patients with familial polyposis coli

Mieko Okamoto, Masayuki Sasaki, Kenji Sugio, Chieko Sato, Takeo Iwama, Tatsuro Ikeuchi, Akira Tonomura, Takehiko Sasazuki, Michiko Miyaki

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Recent studies have suggested a critical role of specific gene loss in several embryonic tumours1-6 and certain adult cancers 7-12. In retinoblastoma, hemizygosity or homozygosity of a recessive mutant allele results in the loss of normal gene product2-4, and this seems to cause the manifestation of the disorder. Familial polyposis coli (FPC) is a human autosomal dominant trait characterized by numerous adenomatous polyps of the colon and rectum, and a high incidence of colon carcinoma. Karyotype analyses have failed to detect specific deletion or translocation. We report the use of polymorphic DNA markers to look for the somatic loss of heterozygosity at specific loci. Investigation of 38 tumours from 25 FPC patients and 20 porad colon carcinomas from 19 patients, revealed frequent occurrence of allele loss on chromosome 22, with some additional losses on chromosomes 5, 6, 12q and 15. The FPC gene-linked DNA probe C11p11 also detected frequent allele loss in both familial and sporadic colon carcinomas but not in benign adenomas. These results suggest the possible involvement of more than one chromosomal locus in the development of familial and sporadic colon carcinomas.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalNature
Volume331
Issue number6153
DOIs
Publication statusPublished - Jan 1 1988

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Adenomatous Polyposis Coli
Loss of Heterozygosity
Colon
Carcinoma
Alleles
Adenomatous Polyps
Chromosomes, Human, Pair 15
Chromosomes, Human, Pair 22
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 6
Retinoblastoma
DNA Probes
Karyotype
Genetic Markers
Rectum
Adenoma
Genes
Neoplasms
Incidence

All Science Journal Classification (ASJC) codes

  • General

Cite this

Okamoto, M., Sasaki, M., Sugio, K., Sato, C., Iwama, T., Ikeuchi, T., ... Miyaki, M. (1988). Loss of constitutional heterozygosity in colon carcinoma from patients with familial polyposis coli. Nature, 331(6153), 273-277. https://doi.org/10.1038/331273a0

Loss of constitutional heterozygosity in colon carcinoma from patients with familial polyposis coli. / Okamoto, Mieko; Sasaki, Masayuki; Sugio, Kenji; Sato, Chieko; Iwama, Takeo; Ikeuchi, Tatsuro; Tonomura, Akira; Sasazuki, Takehiko; Miyaki, Michiko.

In: Nature, Vol. 331, No. 6153, 01.01.1988, p. 273-277.

Research output: Contribution to journalArticle

Okamoto, M, Sasaki, M, Sugio, K, Sato, C, Iwama, T, Ikeuchi, T, Tonomura, A, Sasazuki, T & Miyaki, M 1988, 'Loss of constitutional heterozygosity in colon carcinoma from patients with familial polyposis coli', Nature, vol. 331, no. 6153, pp. 273-277. https://doi.org/10.1038/331273a0
Okamoto, Mieko ; Sasaki, Masayuki ; Sugio, Kenji ; Sato, Chieko ; Iwama, Takeo ; Ikeuchi, Tatsuro ; Tonomura, Akira ; Sasazuki, Takehiko ; Miyaki, Michiko. / Loss of constitutional heterozygosity in colon carcinoma from patients with familial polyposis coli. In: Nature. 1988 ; Vol. 331, No. 6153. pp. 273-277.
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