Loss-of-function mutation in bi-functional marine bacterial sialyltransferase

Hitomi Kajiwara, Sakurako Katayama, Yoshimitsu Kakuta, Nozomu Okino, Makoto Ito, Toshiki Mine, Takeshi Yamamoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An α2,3-sialyltransferase produced by Photobacterium phosphoreum JT-ISH-467 is a bi-functional enzyme showing both α2,3-sialyltransferase and α2,3-linkage specific sialidase activity. To date, the crystal structures of several sialyltransferases have been solved, but the roles of amino acid residues around the catalytic site have not been completely clarified. Hence we performed a mutational study using α2,3- sialyltransferase cloned from P. phosphoreum JT-ISH-467 as a model enzyme to study the role of the amino acid residues around the substrate-binding site. It was found that a mutation of the glutamic acid at position 342 in the sialyltransferase resulted in a loss of sialidase activity, although the mutant showed no decrease in sialyltransferase activity. Based on this result, it is strongly expected that the Glu342 of the enzyme is an important amino acid residue for sialidase activity.

Original languageEnglish
Pages (from-to)1639-1644
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number9
DOIs
Publication statusPublished - Oct 1 2012

Fingerprint

Sialyltransferases
Mutation
Neuraminidase
Amino Acids
Enzymes
Photobacterium
Glutamic Acid
Catalytic Domain
Crystal structure
Binding Sites
Substrates

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Loss-of-function mutation in bi-functional marine bacterial sialyltransferase. / Kajiwara, Hitomi; Katayama, Sakurako; Kakuta, Yoshimitsu; Okino, Nozomu; Ito, Makoto; Mine, Toshiki; Yamamoto, Takeshi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 9, 01.10.2012, p. 1639-1644.

Research output: Contribution to journalArticle

Kajiwara, Hitomi ; Katayama, Sakurako ; Kakuta, Yoshimitsu ; Okino, Nozomu ; Ito, Makoto ; Mine, Toshiki ; Yamamoto, Takeshi. / Loss-of-function mutation in bi-functional marine bacterial sialyltransferase. In: Bioscience, Biotechnology and Biochemistry. 2012 ; Vol. 76, No. 9. pp. 1639-1644.
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