Loss of hIRH mRNA expression from premalignant adenomas and malignant cell lines

Nasim Ara Begum, Ahmet Coker, Kenji Shibuta, Richard S. Swanson, Lan Bo Chen, Masaki Mori, Graham F. Barnard

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We recently isolated from differential displays and subsequently cloned a human α-intercrine (hIRH) whose mRNA is reduced in human hepatocellular carcinomas. We now report that on Northern blots its mRNA is absent from premalignant colonic adenomas and from all of 27 human malignant cell lines (including breast, cervix, colon, duodenal, gastric, leukemia, liver, lung, melanoma, and pancreatic lines). hIRH mRNA was present in most normal human and mouse tissues and fibroblast derived cell lines but absent from leukocytes and brain. Two mRNA signals, at ~ 2 Kb and ~ 3.5 Kb, had variation in signal strength or size between tissues and species.

Original languageEnglish
Pages (from-to)864-868
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume229
Issue number3
DOIs
Publication statusPublished - Dec 24 1996

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Adenocarcinoma
Cells
Cell Line
Messenger RNA
Tissue
Fibroblasts
Chemokines
Cervix Uteri
Northern Blotting
Adenoma
Liver
Hepatocellular Carcinoma
Melanoma
Brain
Stomach
Colon
Leukemia
Breast
Leukocytes
Display devices

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Loss of hIRH mRNA expression from premalignant adenomas and malignant cell lines. / Begum, Nasim Ara; Coker, Ahmet; Shibuta, Kenji; Swanson, Richard S.; Chen, Lan Bo; Mori, Masaki; Barnard, Graham F.

In: Biochemical and Biophysical Research Communications, Vol. 229, No. 3, 24.12.1996, p. 864-868.

Research output: Contribution to journalArticle

Begum, Nasim Ara ; Coker, Ahmet ; Shibuta, Kenji ; Swanson, Richard S. ; Chen, Lan Bo ; Mori, Masaki ; Barnard, Graham F. / Loss of hIRH mRNA expression from premalignant adenomas and malignant cell lines. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 229, No. 3. pp. 864-868.
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