Loss of hIRH mRNA expression from premalignant adenomas and malignant cell lines

Nasim Ara Begum, Ahmet Coker, Kenji Shibuta, Richard S. Swanson, Lan Bo Chen, Masaki Mori, Graham F. Barnard

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We recently isolated from differential displays and subsequently cloned a human α-intercrine (hIRH) whose mRNA is reduced in human hepatocellular carcinomas. We now report that on Northern blots its mRNA is absent from premalignant colonic adenomas and from all of 27 human malignant cell lines (including breast, cervix, colon, duodenal, gastric, leukemia, liver, lung, melanoma, and pancreatic lines). hIRH mRNA was present in most normal human and mouse tissues and fibroblast derived cell lines but absent from leukocytes and brain. Two mRNA signals, at ~ 2 Kb and ~ 3.5 Kb, had variation in signal strength or size between tissues and species.

Original languageEnglish
Pages (from-to)864-868
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume229
Issue number3
DOIs
Publication statusPublished - Dec 24 1996

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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