Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli

Ayaka Matsumoto, Chiyomi Sakamoto, Haruka Matsumori, Jun Katahira, Yoko Yasuda, Katsuhide Yoshidome, Masahiko Tsujimoto, Ilya G. Goldberg, Nariaki Matsuura, Mitsuyoshi Nakao, Noriko Saitoh, Miki Hieda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A supervised machine learning algorithm, which is qualified for image classification and analyzing similarities, is based on multiple discriminative morphological features that are automatically assembled during the learning processes. The algorithm is suitable for population-based analysis of images of biological materials that are generally complex and heterogeneous. Here we used the algorithm wndchrm to quantify the effects on nucleolar morphology of the loss of the components of nuclear envelope in a human mammary epithelial cell line. The linker of nucleoskeleton and cytoskeleton (LINC) complex, an assembly of nuclear envelope proteins comprising mainly members of the SUN and nesprin families, connects the nuclear lamina and cytoskeletal filaments. The components of the LINC complex are markedly deficient in breast cancer tissues. We found that a reduction in the levels of SUN1, SUN2, and lamin A/C led to significant changes in morphologies that were computationally classified using wndchrm with approximately 100% accuracy. In particular, depletion of SUN1 caused nucleolar hypertrophy and reduced rRNA synthesis. Further, wndchrm revealed a consistent negative correlation between SUN1 expression and the size of nucleoli in human breast cancer tissues. Our unbiased morphological quantitation strategies using wndchrm revealed an unexpected link between the components of the LINC complex and the morphologies of nucleoli that serves as an indicator of the malignant phenotype of breast cancer cells.

Original languageEnglish
Pages (from-to)68-83
Number of pages16
JournalNucleus
Volume7
Issue number1
DOIs
Publication statusPublished - Mar 1 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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    Matsumoto, A., Sakamoto, C., Matsumori, H., Katahira, J., Yasuda, Y., Yoshidome, K., Tsujimoto, M., Goldberg, I. G., Matsuura, N., Nakao, M., Saitoh, N., & Hieda, M. (2016). Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli. Nucleus, 7(1), 68-83. https://doi.org/10.1080/19491034.2016.1149664