Lotus japonicus nodulation is photomorphogenetically controlled by sensing the red/far red (R/FR) ratio through jasmonic acid (JA) signaling

Akihiro Suzuki, Lalith Suriyagoda, Tamaki Shigeyama, Akiyoshi Tominaga, Masayo Sasaki, Yoshimi Hiratsuka, Aya Yoshinaga, Susumu Arima, Sakae Agarie, Tatsuya Sakai, Sayaka Inada, Yusuke Jikumaru, Yuji Kamiya, Toshiki Uchiumi, Mikiko Abe, Masatsugu Hashiguchi, Ryo Akashi, Shusei Sato, Takakazu Kaneko, Satoshi TabataAnn M. Hirsch

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Abstract

Light is critical for supplying carbon to the energetically expensive, nitrogen-fixing symbiosis between legumes and rhizobia. Here, we show that phytochrome B (phyB) is part of the monitoring system to detect suboptimal light conditions, which normally suppress Lotus japonicus nodule development after Mesorhizobium loti inoculation. We found that the number of nodules produced by L. japonicus phyB mutants is significantly reduced compared with the number produced of WT Miyakojima MG20. To explore causes other than photoassimilate production, the possibility that local control by the root genotype occurred was investigated by grafting experiments. The results showed that the shoot and not the root genotype is responsible for root nodule formation. To explore systemic control mechanisms exclusive of photoassimilation, we movedWTMG20 plants from white light to conditions that differed in their ratios of low or high red/far red (R/FR) light. In low R/FR light, the number of MG20 root nodules dramatically decreased compared with plants grown in high R/FR, although photoassimilate content was higher for plants grown under low R/FR. Also, the expression of jasmonic acid (JA) -responsive genes decreased in both low R/FR light-grownWT and white light-grown phyB mutant plants, and it correlated with decreased jasmonoyl-isoleucine content in the phyB mutant. Moreover, both infection thread formation and root nodule formation were positively influenced by JA treatment of WT plants grown in low R/FR light and white light-grown phyB mutants. Together, these results indicate that root nodule formation is photomorphogenetically controlled by sensing the R/FR ratio through JA signaling.

Original languageEnglish
Pages (from-to)16837-16842
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number40
DOIs
Publication statusPublished - Oct 4 2011

All Science Journal Classification (ASJC) codes

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    Suzuki, A., Suriyagoda, L., Shigeyama, T., Tominaga, A., Sasaki, M., Hiratsuka, Y., Yoshinaga, A., Arima, S., Agarie, S., Sakai, T., Inada, S., Jikumaru, Y., Kamiya, Y., Uchiumi, T., Abe, M., Hashiguchi, M., Akashi, R., Sato, S., Kaneko, T., ... Hirsch, A. M. (2011). Lotus japonicus nodulation is photomorphogenetically controlled by sensing the red/far red (R/FR) ratio through jasmonic acid (JA) signaling. Proceedings of the National Academy of Sciences of the United States of America, 108(40), 16837-16842. https://doi.org/10.1073/pnas.1105892108