Abstract
A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)80 tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture that is quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation. We have evaluated the performance for SPG/poly(dA) tailed DNA and other related complexes.
| Original language | English |
|---|---|
| Title of host publication | 54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan |
| Pages | 2141 |
| Number of pages | 1 |
| Volume | 54 |
| Edition | 1 |
| Publication status | Published - 2005 |
| Event | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: May 25 2005 → May 27 2005 |
Other
| Other | 54th SPSJ Annual Meeting 2005 |
|---|---|
| Country | Japan |
| City | Yokohama |
| Period | 5/25/05 → 5/27/05 |
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All Science Journal Classification (ASJC) codes
- Engineering(all)
Cite this
Low cytotoxic gene delivery system by a polysaccharide-poly(dA) tailed DNA complex. / Sakurai, Kazuo; Anada, Takahisa; Karinaga, Ryouji; Hamada, Emi; Koumoto, Kazuya; Mizu, Masami; Shinkai, Seiji.
54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2141.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Low cytotoxic gene delivery system by a polysaccharide-poly(dA) tailed DNA complex
AU - Sakurai, Kazuo
AU - Anada, Takahisa
AU - Karinaga, Ryouji
AU - Hamada, Emi
AU - Koumoto, Kazuya
AU - Mizu, Masami
AU - Shinkai, Seiji
PY - 2005
Y1 - 2005
N2 - A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)80 tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture that is quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation. We have evaluated the performance for SPG/poly(dA) tailed DNA and other related complexes.
AB - A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)80 tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture that is quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation. We have evaluated the performance for SPG/poly(dA) tailed DNA and other related complexes.
UR - http://www.scopus.com/inward/record.url?scp=33645649716&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645649716&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:33645649716
VL - 54
SP - 2141
BT - 54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan
ER -