Low cytotoxic gene delivery system by a polysaccharide-poly(dA) tailed DNA complex

Kazuo Sakurai; Takahisa Anada; Ryouji Karinaga; Emi Hamada; Kazuya Koumoto; Masami Mizu; Seiji Shinkai

Low cytotoxic gene delivery system by a polysaccharide-poly(dA) tailed DNA complex

Kazuo Sakurai, Takahisa Anada, Ryouji Karinaga, Emi Hamada, Kazuya Koumoto, Masami Mizu, Seiji Shinkai

Institute for Advanced Study

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)₈₀ tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture that is quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation. We have evaluated the performance for SPG/poly(dA) tailed DNA and other related complexes.

Original language	English
Title of host publication	54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan
Pages	2141
Number of pages	1
Volume	54
Edition	1
Publication status	Published - 2005
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: May 25 2005 → May 27 2005

Other

Other	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	5/25/05 → 5/27/05

All Science Journal Classification (ASJC) codes

Engineering(all)

Cite this

Low cytotoxic gene delivery system by a polysaccharide-poly(dA) tailed DNA complex. / Sakurai, Kazuo; Anada, Takahisa; Karinaga, Ryouji; Hamada, Emi; Koumoto, Kazuya; Mizu, Masami; Shinkai, Seiji.

54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2141.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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abstract = "A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)80 tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture that is quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation. We have evaluated the performance for SPG/poly(dA) tailed DNA and other related complexes.",

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AU - Sakurai, Kazuo

AU - Anada, Takahisa

AU - Karinaga, Ryouji

AU - Hamada, Emi

AU - Koumoto, Kazuya

AU - Mizu, Masami

AU - Shinkai, Seiji

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Low cytotoxic gene delivery system by a polysaccharide-poly(dA) tailed DNA complex

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