Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice

Shingo Matsumoto, Fuminori Hyodo, Sankaran Subramanian, Nallathamby Devasahayam, Jeeva Munasinghe, Emi Hyodo, Chandramouli Gadisetti, John A. Cook, James B. Mitchell, Murali C. Krishna

    Research output: Contribution to journalArticle

    84 Citations (Scopus)

    Abstract

    A priori knowledge of spatial and temporal changes in partial pressure of oxygen (oxygenation; pO2) in solid tumors, a key prognostic factor in cancer treatment outcome, could greatly improve treatment planning in radiotherapy and chemotherapy. Pulsed electron paramagnetic resonance imaging (EPRI) provides quantitative 3D maps of tissue pO2 in living objects. In this study, we implemented an EPRI set-up that could acquire pO2 maps in almost real time for 2D and in minutes for 3D. We also designed a combined EPRI and MRI system that enabled generation of pO2 maps with anatomic guidance. Using EPRI and an air/carbogen (95% O2 plus 5% CO2) breathing cycle, we visualized perfusion-limited hypoxia in murine tumors. The relationship between tumor blood perfusion and pO2 status was examined, and it was found that significant hypoxia existed even in regions that exhibited blood flow. In addition, high levels of lactate were identified even in normoxic tumor regions, suggesting the predominance of aerobic glycolysis in murine tumors. This report presents a rapid, noninvasive method to obtain quantitative maps of pO2 in tumors, reported with anatomy, with precision. In addition, this method may also be useful for studying the relationship between pO2 status and tumor-specific phenotypes such as aerobic glycolysis.

    Original languageEnglish
    Pages (from-to)1965-1973
    Number of pages9
    JournalJournal of Clinical Investigation
    Volume118
    Issue number5
    DOIs
    Publication statusPublished - May 1 2008

    Fingerprint

    Electron Spin Resonance Spectroscopy
    Neoplasms
    Glycolysis
    Perfusion
    Partial Pressure
    Lactic Acid
    Anatomy
    Respiration
    Radiotherapy
    Air
    Oxygen
    Phenotype
    Drug Therapy

    All Science Journal Classification (ASJC) codes

    • Medicine(all)

    Cite this

    Matsumoto, S., Hyodo, F., Subramanian, S., Devasahayam, N., Munasinghe, J., Hyodo, E., ... Krishna, M. C. (2008). Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice. Journal of Clinical Investigation, 118(5), 1965-1973. https://doi.org/10.1172/JCI34928

    Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice. / Matsumoto, Shingo; Hyodo, Fuminori; Subramanian, Sankaran; Devasahayam, Nallathamby; Munasinghe, Jeeva; Hyodo, Emi; Gadisetti, Chandramouli; Cook, John A.; Mitchell, James B.; Krishna, Murali C.

    In: Journal of Clinical Investigation, Vol. 118, No. 5, 01.05.2008, p. 1965-1973.

    Research output: Contribution to journalArticle

    Matsumoto, S, Hyodo, F, Subramanian, S, Devasahayam, N, Munasinghe, J, Hyodo, E, Gadisetti, C, Cook, JA, Mitchell, JB & Krishna, MC 2008, 'Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice', Journal of Clinical Investigation, vol. 118, no. 5, pp. 1965-1973. https://doi.org/10.1172/JCI34928
    Matsumoto S, Hyodo F, Subramanian S, Devasahayam N, Munasinghe J, Hyodo E et al. Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice. Journal of Clinical Investigation. 2008 May 1;118(5):1965-1973. https://doi.org/10.1172/JCI34928
    Matsumoto, Shingo ; Hyodo, Fuminori ; Subramanian, Sankaran ; Devasahayam, Nallathamby ; Munasinghe, Jeeva ; Hyodo, Emi ; Gadisetti, Chandramouli ; Cook, John A. ; Mitchell, James B. ; Krishna, Murali C. / Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice. In: Journal of Clinical Investigation. 2008 ; Vol. 118, No. 5. pp. 1965-1973.
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