レタス(Lactuca sativa L.)プロトプラストへのイオンビーム照射によって出現した低ポリフェノールオキシダーゼ活性を示す突然変異体

Translated title of the contribution: Low Polyphenol Oxidase Mutant Induced by <sup>12</sup>C<sup>6+</sup> Ion Beam Irradiation to Protoplasts of Lettuce (<i>Lactuca sativa</i> L.)

澤田 倫平, 平野 智也, 飯牟禮 和彦, 阿部 知子, 尾崎 行生

Research output: Contribution to journalArticlepeer-review

Abstract

<p>The induction of polyphenol oxidase (PPO) gene mutation through ion beam irradiation of protoplasts was attempted for the production of lettuce plants with a low browning property as a cut vegetable. It was clarified that the optimum protoplast density was 0.63–0.75 × 10<sup>4</sup> mL<sup>–1</sup> for shoot regeneration from protoplasts of lettuce 'Round'. Effects of <sup>12</sup>C<sup>6+</sup> ion beam irradiation on the growth of protoplasts were investigated, and the percentage of colony formation decreased as the irradiation dose increased. The dose leading to 50% cell growth as compared with the untreated control was approximately 3.3 Gy. Two out of 869 calli irradiated with <sup>12</sup>C<sup>6+</sup> ion beams were found to be <i>PPO</i> gene mutations by the TILLING method. PPO activities of the regenerated plants from the two mutant calli named 2Gy-3-36 and 5Gy-2-27 were preliminarily assayed, and lettuce plants from 2Gy-3-36 exhibited half of the PPO activity as compared with the non-irradiated controls. A part of the M<sub>2</sub> population from 2Gy-2-36 exhibited the property of no band of heteroduplex cleavage by <i>Cel</i> I, with markedly low PPO activity as the control.</p>
Translated title of the contributionLow Polyphenol Oxidase Mutant Induced by <sup>12</sup>C<sup>6+</sup> Ion Beam Irradiation to Protoplasts of Lettuce (<i>Lactuca sativa</i> L.)
Original languageJapanese
Pages (from-to)347-353
Number of pages7
Journal園芸学研究
Volume15
Issue number4
DOIs
Publication statusPublished - 2016

Fingerprint Dive into the research topics of 'Low Polyphenol Oxidase Mutant Induced by <sup>12</sup>C<sup>6+</sup> Ion Beam Irradiation to Protoplasts of Lettuce (<i>Lactuca sativa</i> L.)'. Together they form a unique fingerprint.

Cite this