Low protein diets posttranscriptionally repress apolipoprotein B expression in rat liver

Masao Sato, Koji Nagao, Masanobu Sakono, Hiroshi Ogawa, Kyosuke Yamamoto, Katsumi Imaizumi

Research output: Contribution to journalArticle

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Abstract

In an attempt to elucidate molecular mechanisms related to low protein diet-induced fatty livers, apolipoprotein (apo) mRNA and its expression in the liver were studied in rats. Rats were fed for 14 days a diet containing 5% and 20% casein, and whey protein equivalent in protein to these caseins. In the protein-deficient groups, liver triacylglycerols elevated to 1.5-2 fold higher than did the diet sufficient groups. The mRNAs for apo A-IV, apo B and apo E were 20 to 50% lower in the deficient groups than in the sufficient groups, but apo A-I mRNA was not reduced. The rate of transcription of apo A-IV, B, and E genes in hepatic nuclei isolated from those animals was not altered with different levels of dietary protein. Apo B concentration in the serum was lowered and correlated positively with apo B mRNA abundance but the concentrations of apo IV and apo E were elevated in the deficient groups. It thus appears that a low protein diet decreases apo B synthesis through a posttranscriptional mechanism and thereby depresses secretion of triacylglycerol-rich lipoproteins from the liver.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalJournal of Nutritional Biochemistry
Volume7
Issue number7
DOIs
Publication statusPublished - Jan 1 1996

Fingerprint

Protein-Restricted Diet
Apolipoproteins B
Nutrition
Liver
Rats
Messenger RNA
Apolipoproteins
Apolipoproteins E
Caseins
Triglycerides
Proteins
Diet
Dietary Proteins
Apolipoprotein A-I
Fatty Liver
Lipoproteins
Transcription
Animals
Genes
Serum

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Low protein diets posttranscriptionally repress apolipoprotein B expression in rat liver. / Sato, Masao; Nagao, Koji; Sakono, Masanobu; Ogawa, Hiroshi; Yamamoto, Kyosuke; Imaizumi, Katsumi.

In: Journal of Nutritional Biochemistry, Vol. 7, No. 7, 01.01.1996, p. 381-385.

Research output: Contribution to journalArticle

Sato, Masao ; Nagao, Koji ; Sakono, Masanobu ; Ogawa, Hiroshi ; Yamamoto, Kyosuke ; Imaizumi, Katsumi. / Low protein diets posttranscriptionally repress apolipoprotein B expression in rat liver. In: Journal of Nutritional Biochemistry. 1996 ; Vol. 7, No. 7. pp. 381-385.
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