Abstract
In an attempt to elucidate molecular mechanisms related to low protein diet-induced fatty livers, apolipoprotein (apo) mRNA and its expression in the liver were studied in rats. Rats were fed for 14 days a diet containing 5% and 20% casein, and whey protein equivalent in protein to these caseins. In the protein-deficient groups, liver triacylglycerols elevated to 1.5-2 fold higher than did the diet sufficient groups. The mRNAs for apo A-IV, apo B and apo E were 20 to 50% lower in the deficient groups than in the sufficient groups, but apo A-I mRNA was not reduced. The rate of transcription of apo A-IV, B, and E genes in hepatic nuclei isolated from those animals was not altered with different levels of dietary protein. Apo B concentration in the serum was lowered and correlated positively with apo B mRNA abundance but the concentrations of apo IV and apo E were elevated in the deficient groups. It thus appears that a low protein diet decreases apo B synthesis through a posttranscriptional mechanism and thereby depresses secretion of triacylglycerol-rich lipoproteins from the liver.
| Original language | English |
|---|---|
| Pages (from-to) | 381-385 |
| Number of pages | 5 |
| Journal | Journal of Nutritional Biochemistry |
| Volume | 7 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jan 1 1996 |
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All Science Journal Classification (ASJC) codes
- Endocrinology, Diabetes and Metabolism
- Biochemistry
- Molecular Biology
- Nutrition and Dietetics
- Clinical Biochemistry
Cite this
Low protein diets posttranscriptionally repress apolipoprotein B expression in rat liver. / Sato, Masao; Nagao, Koji; Sakono, Masanobu; Ogawa, Hiroshi; Yamamoto, Kyosuke; Imaizumi, Katsumi.
In: Journal of Nutritional Biochemistry, Vol. 7, No. 7, 01.01.1996, p. 381-385.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Low protein diets posttranscriptionally repress apolipoprotein B expression in rat liver
AU - Sato, Masao
AU - Nagao, Koji
AU - Sakono, Masanobu
AU - Ogawa, Hiroshi
AU - Yamamoto, Kyosuke
AU - Imaizumi, Katsumi
PY - 1996/1/1
Y1 - 1996/1/1
N2 - In an attempt to elucidate molecular mechanisms related to low protein diet-induced fatty livers, apolipoprotein (apo) mRNA and its expression in the liver were studied in rats. Rats were fed for 14 days a diet containing 5% and 20% casein, and whey protein equivalent in protein to these caseins. In the protein-deficient groups, liver triacylglycerols elevated to 1.5-2 fold higher than did the diet sufficient groups. The mRNAs for apo A-IV, apo B and apo E were 20 to 50% lower in the deficient groups than in the sufficient groups, but apo A-I mRNA was not reduced. The rate of transcription of apo A-IV, B, and E genes in hepatic nuclei isolated from those animals was not altered with different levels of dietary protein. Apo B concentration in the serum was lowered and correlated positively with apo B mRNA abundance but the concentrations of apo IV and apo E were elevated in the deficient groups. It thus appears that a low protein diet decreases apo B synthesis through a posttranscriptional mechanism and thereby depresses secretion of triacylglycerol-rich lipoproteins from the liver.
AB - In an attempt to elucidate molecular mechanisms related to low protein diet-induced fatty livers, apolipoprotein (apo) mRNA and its expression in the liver were studied in rats. Rats were fed for 14 days a diet containing 5% and 20% casein, and whey protein equivalent in protein to these caseins. In the protein-deficient groups, liver triacylglycerols elevated to 1.5-2 fold higher than did the diet sufficient groups. The mRNAs for apo A-IV, apo B and apo E were 20 to 50% lower in the deficient groups than in the sufficient groups, but apo A-I mRNA was not reduced. The rate of transcription of apo A-IV, B, and E genes in hepatic nuclei isolated from those animals was not altered with different levels of dietary protein. Apo B concentration in the serum was lowered and correlated positively with apo B mRNA abundance but the concentrations of apo IV and apo E were elevated in the deficient groups. It thus appears that a low protein diet decreases apo B synthesis through a posttranscriptional mechanism and thereby depresses secretion of triacylglycerol-rich lipoproteins from the liver.
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U2 - 10.1016/S0955-2863(96)00059-9
DO - 10.1016/S0955-2863(96)00059-9
M3 - Article
VL - 7
SP - 381
EP - 385
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
SN - 0955-2863
IS - 7
ER -