Low-Voltage Irreversible Electroporation Using a Comb-Shaped Contact Electrode

Kosaku Kurata, Shuto Yoshimatsu, Hiroshi Takamatsu

Research output: Contribution to journalArticle

Abstract

Objective: Irreversible electroporation (IRE) is a less invasive therapy to ablate tumor cells by delivering short intensive electric pulses more than a few kV via needle-like electrodes. For reducing the required voltage for the IRE, a durable comb-shaped miniature electrode was designed to use in contact with the lesion surface for a new method named contact IRE. Methods: A miniature electrode was newly fabricated by a fine inkjet patterning and the subsequent etching of a copper-clad polyimide film. A train of 10-<formula><tex>$\mu$</tex></formula>s or 100-<formula><tex>$\mu$</tex></formula>s long electric pulses were applied 90 times at the interval of 1 s to a tissue phantom, and its cross section was observed to measure the necrotized area. Results: Cell experiments showed that the maximum ablation depth increased as a function of the applied voltage and reached 400 <formula><tex>$\mu$</tex></formula>m at 20 V. Furthermore, insulation of the lateral space between electrode teeth with a resin and administration of adjuvants to reduce the IRE threshold of the cell membrane did increase the ablation depth by 26 % and the ablation area by 40 %. Conclusion: The miniature electrode developed in this study successfully necrotized cells in a tissue phantom 400 <formula><tex>$\mu$</tex></formula>m deep from the surface with the electric pulses of only 20 V. Significance: The contact IRE for the surface of skin and gastrointestinal tract will ablate cutaneous and subcutaneous tumors by applying only several tens of volts.

Original languageEnglish
JournalIEEE Transactions on Biomedical Engineering
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Ablation
Electrodes
Electric potential
Tumors
Tissue
Cell membranes
Polyimides
Needles
Insulation
Etching
Skin
Resins
Cells
Copper
Experiments

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

Cite this

@article{ee3add3877f2427aa26f0e93a9923f9a,
title = "Low-Voltage Irreversible Electroporation Using a Comb-Shaped Contact Electrode",
abstract = "Objective: Irreversible electroporation (IRE) is a less invasive therapy to ablate tumor cells by delivering short intensive electric pulses more than a few kV via needle-like electrodes. For reducing the required voltage for the IRE, a durable comb-shaped miniature electrode was designed to use in contact with the lesion surface for a new method named contact IRE. Methods: A miniature electrode was newly fabricated by a fine inkjet patterning and the subsequent etching of a copper-clad polyimide film. A train of 10-$\mu$s or 100-$\mu$s long electric pulses were applied 90 times at the interval of 1 s to a tissue phantom, and its cross section was observed to measure the necrotized area. Results: Cell experiments showed that the maximum ablation depth increased as a function of the applied voltage and reached 400 $\mu$m at 20 V. Furthermore, insulation of the lateral space between electrode teeth with a resin and administration of adjuvants to reduce the IRE threshold of the cell membrane did increase the ablation depth by 26 {\%} and the ablation area by 40 {\%}. Conclusion: The miniature electrode developed in this study successfully necrotized cells in a tissue phantom 400 $\mu$m deep from the surface with the electric pulses of only 20 V. Significance: The contact IRE for the surface of skin and gastrointestinal tract will ablate cutaneous and subcutaneous tumors by applying only several tens of volts.",
author = "Kosaku Kurata and Shuto Yoshimatsu and Hiroshi Takamatsu",
year = "2019",
month = "1",
day = "1",
doi = "10.1109/TBME.2019.2914689",
language = "English",
journal = "IEEE Transactions on Biomedical Engineering",
issn = "0018-9294",
publisher = "IEEE Computer Society",

}

TY - JOUR

T1 - Low-Voltage Irreversible Electroporation Using a Comb-Shaped Contact Electrode

AU - Kurata, Kosaku

AU - Yoshimatsu, Shuto

AU - Takamatsu, Hiroshi

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Objective: Irreversible electroporation (IRE) is a less invasive therapy to ablate tumor cells by delivering short intensive electric pulses more than a few kV via needle-like electrodes. For reducing the required voltage for the IRE, a durable comb-shaped miniature electrode was designed to use in contact with the lesion surface for a new method named contact IRE. Methods: A miniature electrode was newly fabricated by a fine inkjet patterning and the subsequent etching of a copper-clad polyimide film. A train of 10-$\mu$s or 100-$\mu$s long electric pulses were applied 90 times at the interval of 1 s to a tissue phantom, and its cross section was observed to measure the necrotized area. Results: Cell experiments showed that the maximum ablation depth increased as a function of the applied voltage and reached 400 $\mu$m at 20 V. Furthermore, insulation of the lateral space between electrode teeth with a resin and administration of adjuvants to reduce the IRE threshold of the cell membrane did increase the ablation depth by 26 % and the ablation area by 40 %. Conclusion: The miniature electrode developed in this study successfully necrotized cells in a tissue phantom 400 $\mu$m deep from the surface with the electric pulses of only 20 V. Significance: The contact IRE for the surface of skin and gastrointestinal tract will ablate cutaneous and subcutaneous tumors by applying only several tens of volts.

AB - Objective: Irreversible electroporation (IRE) is a less invasive therapy to ablate tumor cells by delivering short intensive electric pulses more than a few kV via needle-like electrodes. For reducing the required voltage for the IRE, a durable comb-shaped miniature electrode was designed to use in contact with the lesion surface for a new method named contact IRE. Methods: A miniature electrode was newly fabricated by a fine inkjet patterning and the subsequent etching of a copper-clad polyimide film. A train of 10-$\mu$s or 100-$\mu$s long electric pulses were applied 90 times at the interval of 1 s to a tissue phantom, and its cross section was observed to measure the necrotized area. Results: Cell experiments showed that the maximum ablation depth increased as a function of the applied voltage and reached 400 $\mu$m at 20 V. Furthermore, insulation of the lateral space between electrode teeth with a resin and administration of adjuvants to reduce the IRE threshold of the cell membrane did increase the ablation depth by 26 % and the ablation area by 40 %. Conclusion: The miniature electrode developed in this study successfully necrotized cells in a tissue phantom 400 $\mu$m deep from the surface with the electric pulses of only 20 V. Significance: The contact IRE for the surface of skin and gastrointestinal tract will ablate cutaneous and subcutaneous tumors by applying only several tens of volts.

UR - http://www.scopus.com/inward/record.url?scp=85065987847&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065987847&partnerID=8YFLogxK

U2 - 10.1109/TBME.2019.2914689

DO - 10.1109/TBME.2019.2914689

M3 - Article

C2 - 31059422

AN - SCOPUS:85065987847

JO - IEEE Transactions on Biomedical Engineering

JF - IEEE Transactions on Biomedical Engineering

SN - 0018-9294

ER -