lpr T cells have lower ability to maintain bcl-2 expression ex vivo

Y. Matsumoto, T. Shinzato, I. Takai, Y. Kimura, S. Nakai, M. Miwa, K. Fuse, Yasunobu Yoshikai, K. Maeda

    Research output: Contribution to journalArticle

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    Abstract

    Autoimmune-prone lpr mice develop lymphoproliferative disorders, whereas their lymphocytes show accelerated apoptosis in culture. To elucidate whether the bcl-2 protein, a repressor of apoptosis, is critical to the discrepancy between in vivo and in vitro survival, we examined bcl-2 expression in T cells from +/+ and lpr mice during culture. The expression levels of bcl-2 in cultured T cells from lpr mice were significantly down-modulated compared to those from +/+ mice and freshly obtained T cells. Besides, the reduction of bcl-2 protein levels was inhibited in T cells cultured in the presence of T cell receptor (TCR) signalling. These results suggest that lpr T cells might be susceptible to apoptosis in vitro due to down-modulation of bcl-2 by withdrawal of TCR signalling.

    Original languageEnglish
    Pages (from-to)283-288
    Number of pages6
    JournalApoptosis
    Volume2
    Issue number3
    DOIs
    Publication statusPublished - Jan 1 1997

    Fingerprint

    T-cells
    T-Lymphocytes
    Apoptosis
    T-Cell Antigen Receptor
    Repressor Proteins
    Lymphoproliferative Disorders
    Lymphocytes
    Cell culture
    Cultured Cells
    Proteins
    Modulation

    All Science Journal Classification (ASJC) codes

    • Pharmacology
    • Pharmaceutical Science
    • Clinical Biochemistry
    • Cell Biology
    • Biochemistry, medical
    • Cancer Research

    Cite this

    Matsumoto, Y., Shinzato, T., Takai, I., Kimura, Y., Nakai, S., Miwa, M., ... Maeda, K. (1997). lpr T cells have lower ability to maintain bcl-2 expression ex vivo. Apoptosis, 2(3), 283-288. https://doi.org/10.1023/A:1026437120118

    lpr T cells have lower ability to maintain bcl-2 expression ex vivo. / Matsumoto, Y.; Shinzato, T.; Takai, I.; Kimura, Y.; Nakai, S.; Miwa, M.; Fuse, K.; Yoshikai, Yasunobu; Maeda, K.

    In: Apoptosis, Vol. 2, No. 3, 01.01.1997, p. 283-288.

    Research output: Contribution to journalArticle

    Matsumoto, Y, Shinzato, T, Takai, I, Kimura, Y, Nakai, S, Miwa, M, Fuse, K, Yoshikai, Y & Maeda, K 1997, 'lpr T cells have lower ability to maintain bcl-2 expression ex vivo', Apoptosis, vol. 2, no. 3, pp. 283-288. https://doi.org/10.1023/A:1026437120118
    Matsumoto Y, Shinzato T, Takai I, Kimura Y, Nakai S, Miwa M et al. lpr T cells have lower ability to maintain bcl-2 expression ex vivo. Apoptosis. 1997 Jan 1;2(3):283-288. https://doi.org/10.1023/A:1026437120118
    Matsumoto, Y. ; Shinzato, T. ; Takai, I. ; Kimura, Y. ; Nakai, S. ; Miwa, M. ; Fuse, K. ; Yoshikai, Yasunobu ; Maeda, K. / lpr T cells have lower ability to maintain bcl-2 expression ex vivo. In: Apoptosis. 1997 ; Vol. 2, No. 3. pp. 283-288.
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