LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet

Eiji Usukura, Yuhki Yanase, Ayumi Ishijima, Thasaneeya Kuboki, Satoru Kidoaki, Koichi Okamoto, Kaoru Tamada

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.

Original languageEnglish
Article numbere0189708
JournalPloS one
Volume12
Issue number12
DOIs
Publication statusPublished - Dec 2017

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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