Abstract
This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.
| Original language | English |
|---|---|
| Article number | e0189708 |
| Journal | PloS one |
| Volume | 12 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 2017 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
Cite this
LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet. / Usukura, Eiji; Yanase, Yuhki; Ishijima, Ayumi; Kuboki, Thasaneeya; Kidoaki, Satoru; Okamoto, Koichi; Tamada, Kaoru.
In: PloS one, Vol. 12, No. 12, e0189708, 12.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet
AU - Usukura, Eiji
AU - Yanase, Yuhki
AU - Ishijima, Ayumi
AU - Kuboki, Thasaneeya
AU - Kidoaki, Satoru
AU - Okamoto, Koichi
AU - Tamada, Kaoru
PY - 2017/12
Y1 - 2017/12
N2 - This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.
AB - This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.
UR - http://www.scopus.com/inward/record.url?scp=85038411903&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85038411903&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0189708
DO - 10.1371/journal.pone.0189708
M3 - Article
C2 - 29244869
AN - SCOPUS:85038411903
VL - 12
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 12
M1 - e0189708
ER -