TY - JOUR
T1 - LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet
AU - Usukura, Eiji
AU - Yanase, Yuhki
AU - Ishijima, Ayumi
AU - Kuboki, Thasaneeya
AU - Kidoaki, Satoru
AU - Okamoto, Koichi
AU - Tamada, Kaoru
N1 - Funding Information:
This work was supported by the NEXT Program and by Japan Society for the Promotion of Science (JSPS) KAKENHI grant number 26246005 in Japan (URL: https://kaken.nii.ac.jp/ grant/KAKENHI-PROJECT-26246005/) (Principal Investigator is Kaoru Tamada); and Ministry of Education, Culture, Sports, Science and Technology (Network Joint Research Center for Materials and Devices) to Yuhki Yanase. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This work was supported by the NEXT Program and by JSPS KAKENHI grant number 26246005 in Japan. This work was performed under the Cooperative Research Program of the “Network Joint Research Center for Materials and Devices”.
PY - 2017/12
Y1 - 2017/12
N2 - This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.
AB - This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.
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U2 - 10.1371/journal.pone.0189708
DO - 10.1371/journal.pone.0189708
M3 - Article
C2 - 29244869
AN - SCOPUS:85038411903
VL - 12
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 12
M1 - e0189708
ER -