LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet

Eiji Usukura, Yuhki Yanase, Ayumi Ishijima, Thasaneeya Kuboki, Satoru Kidoaki, Koichi Okamoto, Kaoru Tamada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.

Original languageEnglish
Article numbere0189708
JournalPloS one
Volume12
Issue number12
DOIs
Publication statusPublished - Dec 2017

Fingerprint

nanosilver
surface plasmon resonance
Surface Plasmon Resonance
Optical Imaging
Surface plasmon resonance
Silver
Nanoparticles
Fluorescence
image analysis
fluorescence
Imaging techniques
Microscopy
microscopy
Microscopic examination
Focal Adhesions
Signal-To-Noise Ratio
cells
Cytotoxicity
Image quality
adhesion

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet. / Usukura, Eiji; Yanase, Yuhki; Ishijima, Ayumi; Kuboki, Thasaneeya; Kidoaki, Satoru; Okamoto, Koichi; Tamada, Kaoru.

In: PloS one, Vol. 12, No. 12, e0189708, 12.2017.

Research output: Contribution to journalArticle

Usukura, Eiji ; Yanase, Yuhki ; Ishijima, Ayumi ; Kuboki, Thasaneeya ; Kidoaki, Satoru ; Okamoto, Koichi ; Tamada, Kaoru. / LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet. In: PloS one. 2017 ; Vol. 12, No. 12.
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