Luminescence of cup-stacked carbon nanotubes and its application to microchip electrophoresis

Yousuke Inoue, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticle

Abstract

Microchip electrophoresis (MCE) is one of the most suitable methods for biomolecular separation and analysis because of its superior characteristics. In biomolecular separation and analysis, laser induced fluorescent (LIF) detection is frequently employed in MCE and attains high sensitive detection. However, LIF detection requires the labeling of analytes, which contains troublesome procedures and results in decreasing of the separation efficiency. In addition, the labeling reaction at low concentration is significantly difficult, and thus true high sensitive detection is not easily attained and has been desired. Here, we report on the possibility of cup-stacked carbon nanotubes (CSCNTs) for high sensitive label-free detection and high separation efficiency. At first we investigated fluorescence properties of CSCNTs. Our investigation revealed that supernatant solution of CSCNTs after centrifugation had fluorescence at around 500 nm, while CSCNTs suspension did not. The application of CSCNTs for MCE enabled us to successfully separate and detect DNA without labeling it. Compared to the direct detection method, our method resulted in poor separation efficiency. However, by controlling of the CSCNTs' aspect ratio and the immobilization of functional molecules on the CSCNTs surfaces, we could improve separation efficiency and attain the separation of various samples without labeling samples.

Original languageEnglish
Pages (from-to)517-521
Number of pages5
Journalbunseki kagaku
Volume58
Issue number6
DOIs
Publication statusPublished - Dec 24 2009

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Carbon Nanotubes
Electrophoresis
Luminescence
Labeling
Fluorescence
Centrifugation
Lasers
Labels
Aspect ratio
Suspensions
Molecules
DNA

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Luminescence of cup-stacked carbon nanotubes and its application to microchip electrophoresis. / Inoue, Yousuke; Okamoto, Yukihiro; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

In: bunseki kagaku, Vol. 58, No. 6, 24.12.2009, p. 517-521.

Research output: Contribution to journalArticle

Inoue, Yousuke ; Okamoto, Yukihiro ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Luminescence of cup-stacked carbon nanotubes and its application to microchip electrophoresis. In: bunseki kagaku. 2009 ; Vol. 58, No. 6. pp. 517-521.
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