TY - JOUR
T1 - Luminescent europium sensors for specific detection of 8-oxo-dGTP by time-gated fluorescence
AU - Fuchi, Yasufumi
AU - Fukuda, Takashi
AU - Sasaki, Shigeki
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research (B) (Grant Number 15H04633 for S.S.) and a Grant-in-Aid for Scientific Research (C) (Grant Number 17K08212 for Y.F.) from the Japan Society for the Promotion Sciences ( JSPS ). Y.F. also acknowledges support from the Takeda Science Foundation – Japan. We thank Prof. Dr. Nobuhiro Yanai and Masashi Hosoyamada (Kyushu University) for measuring the luminescence lifetimes. We also appreciate The Research Support Centre, Graduate School of Medical Sciences, Kyushu University for the time-gated fluorescence and fluorescence microscopy analyses.
Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research (B) (Grant Number 15H04633 for S.S.) and a Grant-in-Aid for Scientific Research (C) (Grant Number 17K08212 for Y.F.) from the Japan Society for the Promotion Sciences (JSPS). Y.F. also acknowledges support from the Takeda Science Foundation ? Japan. We thank Prof. Dr. Nobuhiro Yanai and Masashi Hosoyamada (Kyushu University) for measuring the luminescence lifetimes. We also appreciate The Research Support Centre, Graduate School of Medical Sciences, Kyushu University for the time-gated fluorescence and fluorescence microscopy analyses.
PY - 2018/7/23
Y1 - 2018/7/23
N2 - The 9-hydroxy-1,3-diazaphenoxazine-2-one unit was conjugated with the Eu3+-cyclen complex through a linker. This diazaphenoxazine group was expected as an antenna unit for the excitation of europium ion, and a selective recognition site for 8-oxo-dGTP base. Among the synthesized three derivatives, the highest fluorescence emission was obtained by the complex constructed of an ethylene linker and the cyclen unit with three N,N-dimethylacetamide groups. The Eu3+-cyclen complex exhibited a selective response to the 8-oxo-dGTP in aqueous media by a time-resolved fluorescence assay.
AB - The 9-hydroxy-1,3-diazaphenoxazine-2-one unit was conjugated with the Eu3+-cyclen complex through a linker. This diazaphenoxazine group was expected as an antenna unit for the excitation of europium ion, and a selective recognition site for 8-oxo-dGTP base. Among the synthesized three derivatives, the highest fluorescence emission was obtained by the complex constructed of an ethylene linker and the cyclen unit with three N,N-dimethylacetamide groups. The Eu3+-cyclen complex exhibited a selective response to the 8-oxo-dGTP in aqueous media by a time-resolved fluorescence assay.
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U2 - 10.1016/j.bmc.2018.04.052
DO - 10.1016/j.bmc.2018.04.052
M3 - Article
C2 - 29731311
AN - SCOPUS:85046708488
VL - 26
SP - 3254
EP - 3260
JO - Bioorganic and Medicinal Chemistry
JF - Bioorganic and Medicinal Chemistry
SN - 0968-0896
IS - 12
ER -