Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD

M. Ichiba, J. Zhang, Chikako Kiyohara, Yoichi Nakanishi, K. Takayama, N. Hara, M. Enoki, K. Tomokuni

Research output: Contribution to journalArticle

Abstract

The effect of genetic polymorphism of DNA repair enzyme on the DNA adduct levels was evaluated in this study. We explored the relationship between polymorphism in the nucleotide excision repair enzyme XPD and DNA adduct levels in lymphocytes. Lymphocyte DNA adducts were measured by a 32P-postlabelling method from 42 subjects. XPD ex23 genotypes were identified by the PCR-RFLP method. Subjects with the XPD ex23 heterozygous genotype (AC) showed significantly lower DNA adduct levels (0.63±0.19 per 108 nucleotides, n=6) than those (0.92±0.38, n=36) with major homozygous genotypes (AA). This result suggests that the genotype of the repair enzyme may be one of the important determinants for DNA adduct levels.

Original languageEnglish
Pages (from-to)289-293
Number of pages5
JournalBiomarkers
Volume6
Issue number4
DOIs
Publication statusPublished - Jul 24 2001

Fingerprint

DNA Repair Enzymes
Lymphocytes
DNA Adducts
Polymorphism
Genotype
Repair
Nucleotides
Genetic Polymorphisms
Enzymes
DNA Repair
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Health, Toxicology and Mutagenesis

Cite this

Ichiba, M., Zhang, J., Kiyohara, C., Nakanishi, Y., Takayama, K., Hara, N., ... Tomokuni, K. (2001). Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD. Biomarkers, 6(4), 289-293. https://doi.org/10.1080/13547500010009591

Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD. / Ichiba, M.; Zhang, J.; Kiyohara, Chikako; Nakanishi, Yoichi; Takayama, K.; Hara, N.; Enoki, M.; Tomokuni, K.

In: Biomarkers, Vol. 6, No. 4, 24.07.2001, p. 289-293.

Research output: Contribution to journalArticle

Ichiba, M, Zhang, J, Kiyohara, C, Nakanishi, Y, Takayama, K, Hara, N, Enoki, M & Tomokuni, K 2001, 'Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD', Biomarkers, vol. 6, no. 4, pp. 289-293. https://doi.org/10.1080/13547500010009591
Ichiba M, Zhang J, Kiyohara C, Nakanishi Y, Takayama K, Hara N et al. Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD. Biomarkers. 2001 Jul 24;6(4):289-293. https://doi.org/10.1080/13547500010009591
Ichiba, M. ; Zhang, J. ; Kiyohara, Chikako ; Nakanishi, Yoichi ; Takayama, K. ; Hara, N. ; Enoki, M. ; Tomokuni, K. / Lymphocyte DNA adducts and polymorphism in the DNA repair enzyme XPD. In: Biomarkers. 2001 ; Vol. 6, No. 4. pp. 289-293.
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