Lysosomal acid phosphatase is transported via endosomes to lysosomes

Yoshitaka Tanaka, Shinji Yano, Kazutaka Okada, Toyoko Ishikawa, Masaru Himeno, Keitaro Kato

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Involvement of endosomes in transport of newly synthesized acid phosphatase to lysosomes was investigated using the Golgi fraction (GF1+2), enriched in endosomes. The Golgi fraction (GF1+2) was prepared from the livers of rats given [35S]methionine and asialofetuin conjugated-horseradish peroxidase (HRP). Newly synthesized acid phosphatase in the endosomes containing internalized asialofetuin-HRP was measured as a loss of the detectable labeled enzyme after 3,3′-diaminobenzidine (DAB) and H2O2 reaction, due to formation of insoluble polymers which reduce protein antigenicity. With this procedure, acid phosphatase was all but undetectable in the Golgi fraction. Thus, newly synthesized acid phosphatase is apparently transported to lysosomes by endosomes.

Original languageEnglish
Pages (from-to)1176-1182
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume166
Issue number3
DOIs
Publication statusPublished - Feb 14 1990

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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