M-CSF accelerates neointimal formation in the early phase after vascular injury in mice: The critical role of the SDF-1-CXCR4 system

Yuji Shiba, Masafumi Takahashi, Toru Yoshioka, Noriyuki Yajima, Hajime Morimoto, Atsushi Izawa, Hirohiko Ise, Kiyohiko Hatake, Kazuo Motoyoshi, Uichi Ikeda

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Abstract

OBJECTIVE - Since the macrophage colony-stimulating factor (M-CSF) has been shown to stimulate differentiation and proliferation of monocyte/macrophage lineage and to be involved in the process of neointimal formation after vascular injury, we tested the effects of M-CSF on the recruitment of bone marrow-derived progenitor cells in neointimal formation after vascular injury in mice. METHODS AND RESULTS - Wire-mediated vascular injury was produced in the femoral artery of C57BL/6 mice. Recombinant human M-CSF [500 μg/(kg•day)] or saline (control) was administered for 10 consecutive days, starting 4 days before the injury. Treatment with M-CSF accelerated neointimal formation in the early phase after injury, and this neointimal lesion mainly consisted of bone marrow-derived cells. M-CSF treatment had no effect on the mobilization of endothelial progenitor cells (EPCs: CD34/Flk-1) and reendothelialization after injury. The stromal cell-derived factor-1 (SDF-1) was markedly expressed in the neointima and media after injury, whereas CXCR4 cells were observed in the neointima. Further, a novel CXCR4 antagonist, AMD3100, significantly attenuated the M-CSF-induced neointimal formation. CONCLUSIONS - These findings suggest that M-CSF accelerated neointimal formation after vascular injury via the SDF-1-CXCR4 system, and the inhibition of this system has therapeutic potential for the treatment of cardiovascular diseases.

Original languageEnglish
Pages (from-to)283-289
Number of pages7
JournalArteriosclerosis, thrombosis, and vascular biology
Volume27
Issue number2
DOIs
Publication statusPublished - Feb 1 2007

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Chemokine CXCL12
Macrophage Colony-Stimulating Factor
Vascular System Injuries
Neointima
Wounds and Injuries
Femoral Artery
Inbred C57BL Mouse
Bone Marrow Cells
Monocytes
Cardiovascular Diseases
Stem Cells
Bone Marrow
Macrophages

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

M-CSF accelerates neointimal formation in the early phase after vascular injury in mice : The critical role of the SDF-1-CXCR4 system. / Shiba, Yuji; Takahashi, Masafumi; Yoshioka, Toru; Yajima, Noriyuki; Morimoto, Hajime; Izawa, Atsushi; Ise, Hirohiko; Hatake, Kiyohiko; Motoyoshi, Kazuo; Ikeda, Uichi.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 27, No. 2, 01.02.2007, p. 283-289.

Research output: Contribution to journalArticle

Shiba, Yuji ; Takahashi, Masafumi ; Yoshioka, Toru ; Yajima, Noriyuki ; Morimoto, Hajime ; Izawa, Atsushi ; Ise, Hirohiko ; Hatake, Kiyohiko ; Motoyoshi, Kazuo ; Ikeda, Uichi. / M-CSF accelerates neointimal formation in the early phase after vascular injury in mice : The critical role of the SDF-1-CXCR4 system. In: Arteriosclerosis, thrombosis, and vascular biology. 2007 ; Vol. 27, No. 2. pp. 283-289.
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AU - Yoshioka, Toru

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