Abstract
When mouse bone marrow cells were co-cultured with a stromal cell line. ST2, numerous osteoclast-like multinucleated cells (MNCs) were formed. To enrich the MNCs which were tartrate-resistant acid phosphatase (TRAP)-positive, we treated the cultures with dispase. Enriched TRAP-positive MNCs adhering to the bottom of the dish were cultured for a further 24h with medium containing 15% fetal calf serum. More than 80% of TRAP-positive MNCs were detached from the dish during the culture. However, when ST2 cells were included during the culture period, survival of TRAP-positive MNCs were significantly increased. Moreover, among various factors examined for the survival of TRAP-positive MNCs, only M-CSF and IL-1 alpha were effective in prolonging viability of TRAP-positive MNCs.
| Original language | English |
|---|---|
| Pages (from-to) | 808-811 |
| Number of pages | 4 |
| Journal | Endocrinology |
| Volume | 136 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 1995 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Endocrinology
Cite this
Macrophage colony-stimulating factor and interleukin-1 alpha maintain the survival of osteoclast-like cells. / Jimi, Eijiro; Shuto, Toshihide; Koga, Toshitaka.
In: Endocrinology, Vol. 136, No. 2, 01.01.1995, p. 808-811.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Macrophage colony-stimulating factor and interleukin-1 alpha maintain the survival of osteoclast-like cells
AU - Jimi, Eijiro
AU - Shuto, Toshihide
AU - Koga, Toshitaka
PY - 1995/1/1
Y1 - 1995/1/1
N2 - When mouse bone marrow cells were co-cultured with a stromal cell line. ST2, numerous osteoclast-like multinucleated cells (MNCs) were formed. To enrich the MNCs which were tartrate-resistant acid phosphatase (TRAP)-positive, we treated the cultures with dispase. Enriched TRAP-positive MNCs adhering to the bottom of the dish were cultured for a further 24h with medium containing 15% fetal calf serum. More than 80% of TRAP-positive MNCs were detached from the dish during the culture. However, when ST2 cells were included during the culture period, survival of TRAP-positive MNCs were significantly increased. Moreover, among various factors examined for the survival of TRAP-positive MNCs, only M-CSF and IL-1 alpha were effective in prolonging viability of TRAP-positive MNCs.
AB - When mouse bone marrow cells were co-cultured with a stromal cell line. ST2, numerous osteoclast-like multinucleated cells (MNCs) were formed. To enrich the MNCs which were tartrate-resistant acid phosphatase (TRAP)-positive, we treated the cultures with dispase. Enriched TRAP-positive MNCs adhering to the bottom of the dish were cultured for a further 24h with medium containing 15% fetal calf serum. More than 80% of TRAP-positive MNCs were detached from the dish during the culture. However, when ST2 cells were included during the culture period, survival of TRAP-positive MNCs were significantly increased. Moreover, among various factors examined for the survival of TRAP-positive MNCs, only M-CSF and IL-1 alpha were effective in prolonging viability of TRAP-positive MNCs.
UR - http://www.scopus.com/inward/record.url?scp=0028936354&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028936354&partnerID=8YFLogxK
U2 - 10.1210/endo.136.2.7835314
DO - 10.1210/endo.136.2.7835314
M3 - Article
C2 - 7835314
AN - SCOPUS:0028936354
VL - 136
SP - 808
EP - 811
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 2
ER -