Abstract
When mouse bone marrow cells were co-cultured with a stromal cell line. ST2, numerous osteoclast-like multinucleated cells (MNCs) were formed. To enrich the MNCs which were tartrate-resistant acid phosphatase (TRAP)-positive, we treated the cultures with dispase. Enriched TRAP-positive MNCs adhering to the bottom of the dish were cultured for a further 24h with medium containing 15% fetal calf serum. More than 80% of TRAP-positive MNCs were detached from the dish during the culture. However, when ST2 cells were included during the culture period, survival of TRAP-positive MNCs were significantly increased. Moreover, among various factors examined for the survival of TRAP-positive MNCs, only M-CSF and IL-1 alpha were effective in prolonging viability of TRAP-positive MNCs.
Original language | English |
---|---|
Pages (from-to) | 808-811 |
Number of pages | 4 |
Journal | Endocrinology |
Volume | 136 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 1995 |
All Science Journal Classification (ASJC) codes
- Endocrinology