Macrophage colony-stimulating factor and interleukin-1 alpha maintain the survival of osteoclast-like cells

Eijiro Jimi, Toshihide Shuto, Toshitaka Koga

Research output: Contribution to journalArticlepeer-review

156 Citations (Scopus)

Abstract

When mouse bone marrow cells were co-cultured with a stromal cell line. ST2, numerous osteoclast-like multinucleated cells (MNCs) were formed. To enrich the MNCs which were tartrate-resistant acid phosphatase (TRAP)-positive, we treated the cultures with dispase. Enriched TRAP-positive MNCs adhering to the bottom of the dish were cultured for a further 24h with medium containing 15% fetal calf serum. More than 80% of TRAP-positive MNCs were detached from the dish during the culture. However, when ST2 cells were included during the culture period, survival of TRAP-positive MNCs were significantly increased. Moreover, among various factors examined for the survival of TRAP-positive MNCs, only M-CSF and IL-1 alpha were effective in prolonging viability of TRAP-positive MNCs.

Original languageEnglish
Pages (from-to)808-811
Number of pages4
JournalEndocrinology
Volume136
Issue number2
DOIs
Publication statusPublished - Feb 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Endocrinology

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