Macrophage-colony stimulating factor in obese adipose tissue: Studies with heterozygous op/+ mice

Satoshi Sugita, Yasutomi Kamei, Jun Ichiro Oka, Takayoshi Suganami, Yoshihiro Ogawa

Research output: Contribution to journalArticle

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Abstract

Objective: We examined the gene expression of macrophage-colony stimulating factor (M-CSF) in mice with diet-induced obesity and in genetically obese mice. We also examined the effect of decreased M-CSF signaling on the susceptibility to obesity and macrophage recruitment into the adipose tissue of mice. Research Methods and Procedures: The adipose tissue from mice with diet-induced obesity, obese KKAy mice, and ob/ob obese mice was used for RNA preparation. Production of M-CSF and monocyte chemoattractant protein-1 (MCP-1) was examined by quantitative real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay. The op/+ heterozygous mice, with decreased functional M-CSF expression, were placed on a high-fat diet or crossed with KKAy mice to study the susceptibility to obesity. The gene expression of macrophage markers in adipose tissue was examined.Results: The expression of M-CSF was not significantly changed in mice on a high-fat diet or in either type of genetic obesity (KKAy or ob/ob mice). No change in the degree of obesity or macrophage-related gene expression (F4/80, CD68, and MCP-1) in the adipose tissue was observed in op/+ mice compared with +/+ control mice, which were either treated with a high-fat diet or crossed with KKAy mice. Discussion: This study demonstrated that there was no significant change in the expression of M-CSF in the adipose tissue from obese mice and only a minor phenotypic change, such as macrophage infiltration, in the adipose tissue from op/+ mice, suggesting that M-CSF does not play a major role in macrophage recruitment in the adipose tissue of obese mice.

Original languageEnglish
Pages (from-to)1988-1995
Number of pages8
JournalObesity
Volume15
Issue number8
DOIs
Publication statusPublished - Aug 1 2007
Externally publishedYes

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Macrophage Colony-Stimulating Factor
Adipose Tissue
Obese Mice
Obesity
Macrophages
High Fat Diet
Chemokine CCL2
Gene Expression
Diet
Real-Time Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay
RNA

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

Cite this

Macrophage-colony stimulating factor in obese adipose tissue : Studies with heterozygous op/+ mice. / Sugita, Satoshi; Kamei, Yasutomi; Oka, Jun Ichiro; Suganami, Takayoshi; Ogawa, Yoshihiro.

In: Obesity, Vol. 15, No. 8, 01.08.2007, p. 1988-1995.

Research output: Contribution to journalArticle

Sugita, Satoshi ; Kamei, Yasutomi ; Oka, Jun Ichiro ; Suganami, Takayoshi ; Ogawa, Yoshihiro. / Macrophage-colony stimulating factor in obese adipose tissue : Studies with heterozygous op/+ mice. In: Obesity. 2007 ; Vol. 15, No. 8. pp. 1988-1995.
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