TY - JOUR
T1 - Macrophage foam cell formation is augmented in serum from patients with diabetic angiopathy
AU - Cui, Xinglong
AU - Kushiyama, Akifumi
AU - Yoneda, Masayasu
AU - Nakatsu, Yusuke
AU - Guo, Ying
AU - Zhang, Jun
AU - Ono, Haruya
AU - Kanna, Machi
AU - Sakoda, Hideyuki
AU - Ono, Hiraku
AU - Kikuchi, Takako
AU - Fujishiro, Midori
AU - Shiomi, Masashi
AU - Kamata, Hideaki
AU - Kurihara, Hiroki
AU - Kikuchi, Masatoshi
AU - Kawazu, Shoji
AU - Nishimura, Fusanori
AU - Asano, Tomoichiro
N1 - Funding Information:
We are deeply grateful to Hirokazu Sato and Yuko Oki for their technical supports. This work was supported by a Grant-in-Aid for Exploratory Research from the Japan Promotion of Science Foundation of the Japanese Government.
PY - 2010/1
Y1 - 2010/1
N2 - The differentiation of macrophages into cytokine-secreting foam cells plays a critical role in the development of diabetic angiopathy. J774.1, a murine macrophage cell line, reportedly differentiates into foam cells when incubated with oxidized LDL, ApoE-rich VLDL or WHHLMI (myocardial infarction-prone Watanabe heritable hyperlipidemic) rabbit serum. In this study, serum samples from Type 2 diabetic patients were added to the medium with J774.1 cells and the degree of foam cell induction was quantified by measuring lipid accumulation. These values were calculated relative to the activities of normal and WHHLMI rabbit sera as 0% and 100%, respectively, and termed the MMI (Macrophage Maturation Index). These MMI values reflected intracellular lipids, including cholesteryl ester assayed by GC/MS. Statistical analysis revealed MMI to correlate positively and independently with serum triglycerides, the state of diabetic retinopathy, nephropathy and obesity, but negatively with administration of α-glucosidase inhibitors or thiazolidinediones. Taken together, our results suggest that this novel assay may be applicable to the identification of patients at risk for rapidly progressive angiopathic disorders.
AB - The differentiation of macrophages into cytokine-secreting foam cells plays a critical role in the development of diabetic angiopathy. J774.1, a murine macrophage cell line, reportedly differentiates into foam cells when incubated with oxidized LDL, ApoE-rich VLDL or WHHLMI (myocardial infarction-prone Watanabe heritable hyperlipidemic) rabbit serum. In this study, serum samples from Type 2 diabetic patients were added to the medium with J774.1 cells and the degree of foam cell induction was quantified by measuring lipid accumulation. These values were calculated relative to the activities of normal and WHHLMI rabbit sera as 0% and 100%, respectively, and termed the MMI (Macrophage Maturation Index). These MMI values reflected intracellular lipids, including cholesteryl ester assayed by GC/MS. Statistical analysis revealed MMI to correlate positively and independently with serum triglycerides, the state of diabetic retinopathy, nephropathy and obesity, but negatively with administration of α-glucosidase inhibitors or thiazolidinediones. Taken together, our results suggest that this novel assay may be applicable to the identification of patients at risk for rapidly progressive angiopathic disorders.
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U2 - 10.1016/j.diabres.2009.10.011
DO - 10.1016/j.diabres.2009.10.011
M3 - Article
C2 - 19939487
AN - SCOPUS:73749088655
SN - 0168-8227
VL - 87
SP - 57
EP - 63
JO - Diabetes Research and Clinical Practice
JF - Diabetes Research and Clinical Practice
IS - 1
ER -
