TY - JOUR
T1 - Macrophage inflammatory protein-1α (LD78) expressed in human bone marrow
T2 - Its role in regulation of hematopoiesis and osteoclast recruitment
AU - Kukita, Toshio
AU - Nomiyama, Hisayuki
AU - Ohmoto, Yasukazu
AU - Kukita, Akiko
AU - Shuto, Toshihide
AU - Hotokebuchi, Takao
AU - Sugioka, Yoichi
AU - Miura, Retsu
AU - Iijima, Tadahiko
PY - 1997/3/1
Y1 - 1997/3/1
N2 - Human macrophage inflammatory protein-1α (hMIP-1α), also known as LD78, is a member of the chemokine/intercrine family and an inhibitor of the proliferation of the hematopoietic stem cells in vitro. Using a specific monoclonal antibody, we observed significant localization of hMIP-1α in eosinophilic myelocytes in human bone marrow. We further examined the expression of hMIP-1α mRNA in human bone tissue by in situ hybridization. A high level of hMIP-1α mRNA expression was detected in eosinophilic myelocytes in bone marrow, confirming these cells as the site of hMIP-1α synthesis, hMIP-1α mRNA expression was also detected in osteoblasts in the bone-remodeling sites, and osteoclasts were frequently observed in the vicinity of these osteoblasts, hMIP-1α was also able to induce osteoclastogenesis on calcified matrices in the absence of any other osteotropic hormones. These results strongly suggest that hMIP-1α is involved not only in the regulation of hematopoiesis but also in the modulation of bone remodeling.
AB - Human macrophage inflammatory protein-1α (hMIP-1α), also known as LD78, is a member of the chemokine/intercrine family and an inhibitor of the proliferation of the hematopoietic stem cells in vitro. Using a specific monoclonal antibody, we observed significant localization of hMIP-1α in eosinophilic myelocytes in human bone marrow. We further examined the expression of hMIP-1α mRNA in human bone tissue by in situ hybridization. A high level of hMIP-1α mRNA expression was detected in eosinophilic myelocytes in bone marrow, confirming these cells as the site of hMIP-1α synthesis, hMIP-1α mRNA expression was also detected in osteoblasts in the bone-remodeling sites, and osteoclasts were frequently observed in the vicinity of these osteoblasts, hMIP-1α was also able to induce osteoclastogenesis on calcified matrices in the absence of any other osteotropic hormones. These results strongly suggest that hMIP-1α is involved not only in the regulation of hematopoiesis but also in the modulation of bone remodeling.
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M3 - Article
C2 - 9121122
AN - SCOPUS:0030903039
VL - 76
SP - 399
EP - 406
JO - Laboratory Investigation
JF - Laboratory Investigation
SN - 0023-6837
IS - 3
ER -