Macrophage inflammatory protein-1α (LD78) expressed in human bone marrow: Its role in regulation of hematopoiesis and osteoclast recruitment

Toshio Kukita, Hisayuki Nomiyama, Yasukazu Ohmoto, Akiko Kukita, Toshihide Shuto, Takao Hotokebuchi, Yoichi Sugioka, Retsu Miura, Tadahiko Iijima

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80 Citations (Scopus)

Abstract

Human macrophage inflammatory protein-1α (hMIP-1α), also known as LD78, is a member of the chemokine/intercrine family and an inhibitor of the proliferation of the hematopoietic stem cells in vitro. Using a specific monoclonal antibody, we observed significant localization of hMIP-1α in eosinophilic myelocytes in human bone marrow. We further examined the expression of hMIP-1α mRNA in human bone tissue by in situ hybridization. A high level of hMIP-1α mRNA expression was detected in eosinophilic myelocytes in bone marrow, confirming these cells as the site of hMIP-1α synthesis, hMIP-1α mRNA expression was also detected in osteoblasts in the bone-remodeling sites, and osteoclasts were frequently observed in the vicinity of these osteoblasts, hMIP-1α was also able to induce osteoclastogenesis on calcified matrices in the absence of any other osteotropic hormones. These results strongly suggest that hMIP-1α is involved not only in the regulation of hematopoiesis but also in the modulation of bone remodeling.

Original languageEnglish
Pages (from-to)399-406
Number of pages8
JournalLaboratory Investigation
Volume76
Issue number3
Publication statusPublished - Mar 1 1997

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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