TY - JOUR
T1 - Macroporous glucomannan spheres for the size-exclusion separation of aqueous macromolecules
AU - Hirayama, Chuichi
AU - Ihara, Hirotaka
AU - Shiba, Masayo
AU - Nakamura, Mitsugu
AU - Motozato, Yoshiaki
AU - Kunitake, Toyoki
PY - 1987
Y1 - 1987
N2 - New spherical gels for the high-speed size-exclusion separation of aqueous macromolecules were developed. The gels were prepared by the "sphering" of glucomannan triacetate (from a Amorphophalus konjac) by means of a suspension and evaporation method, followed by a saponification and cross-linking with epichlorohydrin. By adjusting the preparation conditions, spherical gels were obtained with varying exclusion characteristics. For example, polyoxyethylene with a molecular weight below 103 and above 107 in the absence or in the presence of a diluent during the sphering process, respectively. In addition, the packing exhibited a high sample capacity and good pressure stability. These favourable properties are attributable to the low degree of swelling of the glucomannan spheres. The gel permeation chromatography of phosphate bilayer membranes (aggregate weight ca. 107) was investigate.
AB - New spherical gels for the high-speed size-exclusion separation of aqueous macromolecules were developed. The gels were prepared by the "sphering" of glucomannan triacetate (from a Amorphophalus konjac) by means of a suspension and evaporation method, followed by a saponification and cross-linking with epichlorohydrin. By adjusting the preparation conditions, spherical gels were obtained with varying exclusion characteristics. For example, polyoxyethylene with a molecular weight below 103 and above 107 in the absence or in the presence of a diluent during the sphering process, respectively. In addition, the packing exhibited a high sample capacity and good pressure stability. These favourable properties are attributable to the low degree of swelling of the glucomannan spheres. The gel permeation chromatography of phosphate bilayer membranes (aggregate weight ca. 107) was investigate.
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U2 - 10.1016/S0021-9673(01)86793-5
DO - 10.1016/S0021-9673(01)86793-5
M3 - Article
AN - SCOPUS:0006087580
SN - 0021-9673
VL - 409
SP - 175
EP - 181
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -