Macroporous glucomannan spheres for the size-exclusion separation of aqueous macromolecules

Chuichi Hirayama, Hirotaka Ihara, Masayo Shiba, Mitsugu Nakamura, Yoshiaki Motozato, Toyoki Kunitake

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

New spherical gels for the high-speed size-exclusion separation of aqueous macromolecules were developed. The gels were prepared by the "sphering" of glucomannan triacetate (from a Amorphophalus konjac) by means of a suspension and evaporation method, followed by a saponification and cross-linking with epichlorohydrin. By adjusting the preparation conditions, spherical gels were obtained with varying exclusion characteristics. For example, polyoxyethylene with a molecular weight below 103 and above 107 in the absence or in the presence of a diluent during the sphering process, respectively. In addition, the packing exhibited a high sample capacity and good pressure stability. These favourable properties are attributable to the low degree of swelling of the glucomannan spheres. The gel permeation chromatography of phosphate bilayer membranes (aggregate weight ca. 107) was investigate.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalJournal of Chromatography A
Volume409
Issue numberC
DOIs
Publication statusPublished - Jan 1 1987

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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