Mag-seeding of rat bone marrow stromal cells into porous hydroxyapatite scaffolds for bone tissue engineering

Kazunori Shimizu, Akira Ito, Hiroyuki Honda

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Bone tissue engineering has been investigated as an alternative strategy for autograft transplantation. In the process of tissue engineering, cell seeding into three-dimensional (3-D) scaffolds is the first step for constructing 3-D tissues. We have proposed a methodology of cell seeding into 3-D porous scaffolds using magnetic force and magnetite nanoparticles, which we term Mag-seeding. In this study, we applied this Mag-seeding technique to bone tissue engineering using bone marrow stromal cells (BMSCs) and 3-D hydroxyapatite (HA) scaffolds. BMSCs were magnetically labeled with our original magnetite cationic liposomes (MCLs) having a positive surface charge to improve adsorption to cell surface. Magnetically labeled BMSCs were seeded onto a scaffold, and a 1-T magnet was placed under the scaffold. By using Mag-seeding, the cells were successfully seeded into the internal space of scaffolds with a high cell density. The cell seeding efficiency into HA scaffolds by Mag-seeding was approximately threefold larger than that by static-seeding (conventional method, without a magnet). After a 14-d cultivation period using the osteogenic induction medium by Mag-seeding, the level of two representative osteogenic markers (alkaline phosphatase and osteocalcin) were significantly higher than those by static-seeding. These results indicated that Mag-seeding of BMSCs into HA scaffolds is an effective approach to bone tissue engineering.

Original languageEnglish
Pages (from-to)171-177
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume104
Issue number3
DOIs
Publication statusPublished - Sep 1 2007

Fingerprint

Durapatite
Tissue Engineering
Scaffolds (biology)
Mesenchymal Stromal Cells
Hydroxyapatite
Tissue engineering
Scaffolds
Rats
Bone
Bone and Bones
Magnets
Magnetite Nanoparticles
Ferrosoferric Oxide
Osteocalcin
Autografts
Transplantation (surgical)
Liposomes
Magnetite nanoparticles
Adsorption
Alkaline Phosphatase

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering

Cite this

Mag-seeding of rat bone marrow stromal cells into porous hydroxyapatite scaffolds for bone tissue engineering. / Shimizu, Kazunori; Ito, Akira; Honda, Hiroyuki.

In: Journal of Bioscience and Bioengineering, Vol. 104, No. 3, 01.09.2007, p. 171-177.

Research output: Contribution to journalArticle

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