Magnesium decreases arterial pressure and inhibits cardiovascular responses induced by N-methyl-D-aspartate and metabotropic glutamate receptors stimulation in rostral ventrolateral medulla

Shuntaro Kagiyama, Takuya Tsuchihashi, M. Ian Phillips, Isao Abe, Kiyoshi Matsumura, Masatoshi Fujishima

Research output: Contribution to journalArticle

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Abstract

Objective: Magnesium sulfate (MgSO4) is widely used for the treatment of eclampsia. However, effects of Mg2+ in central cardiovascular regulation remain unclear. In the present study, the role of Mg2+ on cardiovascular regulation in the rostral ventrolateral medulla (RVLM) of rats was examined. Methods: Adult male Wistar rats were anesthetized with urethane, and artificially ventilated. The ventral surface of the medulla was exposed, and the RVLM was identified by microinjection (50 nl) of L-glutamate (L-Glu; 2 nmol). Then, MgSO4 (1, 3, 10 nmol, n = 7 for each dose) and magnesium chloride (MgCl2; 10 nmol, n = 7) were microinjected into the RVLM. L-Glu (2 nmol), N-methyl-D-aspartate (NMDA; 20 pmol), α-amino-3-hydroxy-5-methyl isoxazole-4-propionic acid (AMPA; 5 pmol) and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid [(1S,3R)-ACPD, metabotropic glutamate receptor agonist; 1 nmol] were also microinjected with or without pretreatment of MgSO4 (10 nmol; n = 7 for each drug). Results: MgSO4 dose-dependently decreased mean arterial pressure (MAP) and heart rate (HR). The high dose of MgSO4 (10 nmol) significantly decreased MAP and HR (-25 ± 4 mmHg and -43 ± 6 bpm). Similarly, MgCl2 decreased MAP and HR (-27 ± 4 mmHg and -30 ± 6 bpm). The pressor response evoked by NMDA or (1S,3R)-ACPD was significantly attenuated by the pretreatment with MgSO4. In contrast, pressor response caused by L-Glu or AMPA was not affected by pretreatment with MgSO4. Conclusions: These results suggest that Mg2+ has an inhibitory role on the RVLM neurons, and inhibits cardiovascular responses induced by NMDA and metabotropic glutamate receptor agonists.

Original languageEnglish
Pages (from-to)2213-2219
Number of pages7
JournalJournal of hypertension
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 24 2001

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Metabotropic Glutamate Receptors
N-Methylaspartate
Magnesium
Arterial Pressure
Excitatory Amino Acid Agonists
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Magnesium Chloride
Heart Rate
Isoxazoles
Magnesium Sulfate
Eclampsia
Urethane
Microinjections
Wistar Rats
Glutamic Acid
Neurons
Pharmaceutical Preparations
1-amino-1,3-dicarboxycyclopentane
Therapeutics

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Magnesium decreases arterial pressure and inhibits cardiovascular responses induced by N-methyl-D-aspartate and metabotropic glutamate receptors stimulation in rostral ventrolateral medulla. / Kagiyama, Shuntaro; Tsuchihashi, Takuya; Phillips, M. Ian; Abe, Isao; Matsumura, Kiyoshi; Fujishima, Masatoshi.

In: Journal of hypertension, Vol. 19, No. 12, 24.12.2001, p. 2213-2219.

Research output: Contribution to journalArticle

Kagiyama, Shuntaro ; Tsuchihashi, Takuya ; Phillips, M. Ian ; Abe, Isao ; Matsumura, Kiyoshi ; Fujishima, Masatoshi. / Magnesium decreases arterial pressure and inhibits cardiovascular responses induced by N-methyl-D-aspartate and metabotropic glutamate receptors stimulation in rostral ventrolateral medulla. In: Journal of hypertension. 2001 ; Vol. 19, No. 12. pp. 2213-2219.
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abstract = "Objective: Magnesium sulfate (MgSO4) is widely used for the treatment of eclampsia. However, effects of Mg2+ in central cardiovascular regulation remain unclear. In the present study, the role of Mg2+ on cardiovascular regulation in the rostral ventrolateral medulla (RVLM) of rats was examined. Methods: Adult male Wistar rats were anesthetized with urethane, and artificially ventilated. The ventral surface of the medulla was exposed, and the RVLM was identified by microinjection (50 nl) of L-glutamate (L-Glu; 2 nmol). Then, MgSO4 (1, 3, 10 nmol, n = 7 for each dose) and magnesium chloride (MgCl2; 10 nmol, n = 7) were microinjected into the RVLM. L-Glu (2 nmol), N-methyl-D-aspartate (NMDA; 20 pmol), α-amino-3-hydroxy-5-methyl isoxazole-4-propionic acid (AMPA; 5 pmol) and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid [(1S,3R)-ACPD, metabotropic glutamate receptor agonist; 1 nmol] were also microinjected with or without pretreatment of MgSO4 (10 nmol; n = 7 for each drug). Results: MgSO4 dose-dependently decreased mean arterial pressure (MAP) and heart rate (HR). The high dose of MgSO4 (10 nmol) significantly decreased MAP and HR (-25 ± 4 mmHg and -43 ± 6 bpm). Similarly, MgCl2 decreased MAP and HR (-27 ± 4 mmHg and -30 ± 6 bpm). The pressor response evoked by NMDA or (1S,3R)-ACPD was significantly attenuated by the pretreatment with MgSO4. In contrast, pressor response caused by L-Glu or AMPA was not affected by pretreatment with MgSO4. Conclusions: These results suggest that Mg2+ has an inhibitory role on the RVLM neurons, and inhibits cardiovascular responses induced by NMDA and metabotropic glutamate receptor agonists.",
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T1 - Magnesium decreases arterial pressure and inhibits cardiovascular responses induced by N-methyl-D-aspartate and metabotropic glutamate receptors stimulation in rostral ventrolateral medulla

AU - Kagiyama, Shuntaro

AU - Tsuchihashi, Takuya

AU - Phillips, M. Ian

AU - Abe, Isao

AU - Matsumura, Kiyoshi

AU - Fujishima, Masatoshi

PY - 2001/12/24

Y1 - 2001/12/24

N2 - Objective: Magnesium sulfate (MgSO4) is widely used for the treatment of eclampsia. However, effects of Mg2+ in central cardiovascular regulation remain unclear. In the present study, the role of Mg2+ on cardiovascular regulation in the rostral ventrolateral medulla (RVLM) of rats was examined. Methods: Adult male Wistar rats were anesthetized with urethane, and artificially ventilated. The ventral surface of the medulla was exposed, and the RVLM was identified by microinjection (50 nl) of L-glutamate (L-Glu; 2 nmol). Then, MgSO4 (1, 3, 10 nmol, n = 7 for each dose) and magnesium chloride (MgCl2; 10 nmol, n = 7) were microinjected into the RVLM. L-Glu (2 nmol), N-methyl-D-aspartate (NMDA; 20 pmol), α-amino-3-hydroxy-5-methyl isoxazole-4-propionic acid (AMPA; 5 pmol) and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid [(1S,3R)-ACPD, metabotropic glutamate receptor agonist; 1 nmol] were also microinjected with or without pretreatment of MgSO4 (10 nmol; n = 7 for each drug). Results: MgSO4 dose-dependently decreased mean arterial pressure (MAP) and heart rate (HR). The high dose of MgSO4 (10 nmol) significantly decreased MAP and HR (-25 ± 4 mmHg and -43 ± 6 bpm). Similarly, MgCl2 decreased MAP and HR (-27 ± 4 mmHg and -30 ± 6 bpm). The pressor response evoked by NMDA or (1S,3R)-ACPD was significantly attenuated by the pretreatment with MgSO4. In contrast, pressor response caused by L-Glu or AMPA was not affected by pretreatment with MgSO4. Conclusions: These results suggest that Mg2+ has an inhibitory role on the RVLM neurons, and inhibits cardiovascular responses induced by NMDA and metabotropic glutamate receptor agonists.

AB - Objective: Magnesium sulfate (MgSO4) is widely used for the treatment of eclampsia. However, effects of Mg2+ in central cardiovascular regulation remain unclear. In the present study, the role of Mg2+ on cardiovascular regulation in the rostral ventrolateral medulla (RVLM) of rats was examined. Methods: Adult male Wistar rats were anesthetized with urethane, and artificially ventilated. The ventral surface of the medulla was exposed, and the RVLM was identified by microinjection (50 nl) of L-glutamate (L-Glu; 2 nmol). Then, MgSO4 (1, 3, 10 nmol, n = 7 for each dose) and magnesium chloride (MgCl2; 10 nmol, n = 7) were microinjected into the RVLM. L-Glu (2 nmol), N-methyl-D-aspartate (NMDA; 20 pmol), α-amino-3-hydroxy-5-methyl isoxazole-4-propionic acid (AMPA; 5 pmol) and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid [(1S,3R)-ACPD, metabotropic glutamate receptor agonist; 1 nmol] were also microinjected with or without pretreatment of MgSO4 (10 nmol; n = 7 for each drug). Results: MgSO4 dose-dependently decreased mean arterial pressure (MAP) and heart rate (HR). The high dose of MgSO4 (10 nmol) significantly decreased MAP and HR (-25 ± 4 mmHg and -43 ± 6 bpm). Similarly, MgCl2 decreased MAP and HR (-27 ± 4 mmHg and -30 ± 6 bpm). The pressor response evoked by NMDA or (1S,3R)-ACPD was significantly attenuated by the pretreatment with MgSO4. In contrast, pressor response caused by L-Glu or AMPA was not affected by pretreatment with MgSO4. Conclusions: These results suggest that Mg2+ has an inhibitory role on the RVLM neurons, and inhibits cardiovascular responses induced by NMDA and metabotropic glutamate receptor agonists.

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