Magnetic manipulation of a retroviral vector using magnetite cationic liposomes

Akira Ito, Tetsuya Takahashi, Yujiro Kameyama, Kawabe Yoshinori, Masamichi Kamihira

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In the present study, we used magnetite nanoparticles and magnetic force to concentrate the retroviral vectors in order to enhance the transduction efficiency and to enable their magnetic manipulation. Magnetite nanoparticles modified with Cationic liposomes were added to a solution containing a retroviral vector. The magnetic particles which captured the viral vectors were collected by a magnetic force, and seeded into target cells. The viral titer increased up to 55-fold and 3 x 108 IU/mL. Additionally, the magnetically labeled retroviral vectors can be directed to the desired regions for infection by applying magnetic fields, and micro-patterns of gene-transduced cell regions could be created on a cellular monolayer using micro-patterned magnetic concentrators. These results suggest that this new technique provides a promising approach to capture and concentrate viral vectors, thus achieving high transduction efficiency and the ability to deliver genes to a specifically injured site by applying a magnetic field.

Original languageEnglish
Title of host publication2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
Pages367-371
Number of pages5
DOIs
Publication statusPublished - Dec 1 2008
Event2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, Japan
Duration: Nov 6 2008Nov 9 2008

Publication series

Name2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

Other

Other2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
CountryJapan
CityNagoya
Period11/6/0811/9/08

All Science Journal Classification (ASJC) codes

  • Computer Vision and Pattern Recognition
  • Human-Computer Interaction
  • Electrical and Electronic Engineering

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