Magnetic particles-based enzyme immunoassay for rapid determination of secoiridoid glycoside, amarogentin

Seiichi Sakamoto, Shinji Wada, Yui Morita, Tomoko Yamaguchi, Hiroyuki Tanaka, Satoshi Morimoto

Research output: Contribution to journalArticle

Abstract

Amarogentin (AG) is one of the bitter secoiridoid glycosides, which exerts various pharmacological activities as a bitter stomachic. Recently, there is an increasing demand for AG-containing plants in Japan due to their use as folk medicines and food additives; hence, it is crucial to develop analytical techniques that are specific for AG. In this study, a new magnetic particles-based enzyme immunoassay (MPs-EIA) using a specific monoclonal antibody against AG (MAb 1E9) for the rapid determination of AG in plants of the family Gentianaceae was described. AG directly immobilized onto magnetic particles (MPs) was used as a competitor for free AG against MAb 1E9, thereby increasing the surface area of the solid phase and decreasing the immunoreaction time. In addition, the blocking step required in case of the conventional enzyme-linked immunosorbent assay could be avoided in the proposed MPs-EIA, which enables an even more rapid performance for the immunoassay. In the developed MPs-EIA, AG exhibited linearity in the range of 15.6–500 ng mL−1, with a limit of detection of 8.58 ng mL−1. Validation analysis revealed that MPs-EIA is a sufficiently sensitive and rapid for the quantitative analysis of AG in plant samples. To the best of our knowledge, this is the first MPs-EIA that has been applied to plant samples.

LanguageEnglish
Pages731-736
Number of pages6
JournalTalanta
Volume194
DOIs
Publication statusPublished - Mar 1 2019

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Gentianaceae
Iridoid Glycosides
Immunology
Glycosides
Monoclonal antibodies
Allergy and Immunology
Immunoenzyme Techniques
Sugars
Enzymes
Monoclonal Antibodies
Food additives
Medicine
amarogentin
Assays
Food Additives
Immunosorbents
Traditional Medicine
Immunoassay
Limit of Detection
Japan

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry(all)
  • Biochemistry
  • Spectroscopy

Cite this

Magnetic particles-based enzyme immunoassay for rapid determination of secoiridoid glycoside, amarogentin. / Sakamoto, Seiichi; Wada, Shinji; Morita, Yui; Yamaguchi, Tomoko; Tanaka, Hiroyuki; Morimoto, Satoshi.

In: Talanta, Vol. 194, 01.03.2019, p. 731-736.

Research output: Contribution to journalArticle

Sakamoto, Seiichi ; Wada, Shinji ; Morita, Yui ; Yamaguchi, Tomoko ; Tanaka, Hiroyuki ; Morimoto, Satoshi. / Magnetic particles-based enzyme immunoassay for rapid determination of secoiridoid glycoside, amarogentin. In: Talanta. 2019 ; Vol. 194. pp. 731-736.
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