Magnetically triggered transgene expression in mammalian cells by localized cellular heating of magnetic nanoparticles

Akira Ito, Ryoji Teranishi, Kazuki Kamei, Masaki Yamaguchi, Akihiko Ono, Shinya Masumoto, Yuto Sonoda, Masanobu Horie, Kawabe Yoshinori, Masamichi Kamihira

Research output: Contribution to journalArticle

Abstract

To develop a remote control system of transgene expression through localized cellular heating of magnetic nanoparticles, a heat-inducible transgene expression system was introduced into mammalian cells. Cells were labeled with magnetic nanoparticles and exposed to an alternating magnetic field. The magnetically labeled cells expressed the transgene in a monolayer and multilayered cell sheets in which cells were heated around the magnetic nanoparticles without an apparent temperature increase in the culture medium. Magnetic cells were also generated by genetically engineering with a ferritin gene, and transgene expression could be induced by exposure to an alternating magnetic field. This approach may be applicable to the development of novel gene therapies in cell-based medicine.

Original languageEnglish
Pages (from-to)355-364
Number of pages10
JournalJournal of Bioscience and Bioengineering
Volume128
Issue number3
DOIs
Publication statusPublished - Sep 1 2019

Fingerprint

Transgenes
Nanoparticles
Heating
Cells
Magnetic fields
Gene therapy
Ferritins
Remote control
Medicine
Magnetic Fields
Culture Media
Monolayers
Genes
Control systems
Genetic Therapy
Hot Temperature
Temperature
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Magnetically triggered transgene expression in mammalian cells by localized cellular heating of magnetic nanoparticles. / Ito, Akira; Teranishi, Ryoji; Kamei, Kazuki; Yamaguchi, Masaki; Ono, Akihiko; Masumoto, Shinya; Sonoda, Yuto; Horie, Masanobu; Yoshinori, Kawabe; Kamihira, Masamichi.

In: Journal of Bioscience and Bioengineering, Vol. 128, No. 3, 01.09.2019, p. 355-364.

Research output: Contribution to journalArticle

Ito, Akira ; Teranishi, Ryoji ; Kamei, Kazuki ; Yamaguchi, Masaki ; Ono, Akihiko ; Masumoto, Shinya ; Sonoda, Yuto ; Horie, Masanobu ; Yoshinori, Kawabe ; Kamihira, Masamichi. / Magnetically triggered transgene expression in mammalian cells by localized cellular heating of magnetic nanoparticles. In: Journal of Bioscience and Bioengineering. 2019 ; Vol. 128, No. 3. pp. 355-364.
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