TY - JOUR
T1 - Magnetite nanoparticle-loaded anti-HER2 immunoliposomes for combination of antibody therapy with hyperthermia
AU - Ito, Akira
AU - Kuga, Yuko
AU - Honda, Hiroyuki
AU - Kikkawa, Hiroyuki
AU - Horiuchi, Atsushi
AU - Watanabe, Yuji
AU - Kobayashi, Takeshi
N1 - Funding Information:
The authors would like to thank Toda Kogyo Co. for supplying the magnetite. This work was partially supported by a Grant-in-Aid for Scientific Research (No. 13853005), University Start-Ups Creation Support System, and the 21st Century COE Program ‘Nature-Guided Materials Processing’ from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2004/8/30
Y1 - 2004/8/30
N2 - Anti-HER2 antibody can induce antitumor responses, and can be used in delivering drugs to HER2-overexressing cancer. Previously, we produced hyperthermia using magnetite nanoparticles that generate heat in an alternating magnetic field. In the present study, we constructed anti-HER2 immunoliposomes containing magnetite nanoparticles, which act as tumor-targeting vehicles, combining anti-HER2 antibody therapy with hyperthermia. The magnetite nanoparticle-loaded anti-HER2 immunoliposomes exerted HER2-mediated antiproliferative effects on SKBr3 breast cancer cells in vitro. Moreover, 60% of magnetite nanoparticles were incorporated into SKBr3, and the cells were then heated at 42.5°C under an alternating magnetic field, resulting in strong cytotoxic effects. These results suggest that this novel therapeutic tool is applicable to treatment of HER2-overexpressing cancer.
AB - Anti-HER2 antibody can induce antitumor responses, and can be used in delivering drugs to HER2-overexressing cancer. Previously, we produced hyperthermia using magnetite nanoparticles that generate heat in an alternating magnetic field. In the present study, we constructed anti-HER2 immunoliposomes containing magnetite nanoparticles, which act as tumor-targeting vehicles, combining anti-HER2 antibody therapy with hyperthermia. The magnetite nanoparticle-loaded anti-HER2 immunoliposomes exerted HER2-mediated antiproliferative effects on SKBr3 breast cancer cells in vitro. Moreover, 60% of magnetite nanoparticles were incorporated into SKBr3, and the cells were then heated at 42.5°C under an alternating magnetic field, resulting in strong cytotoxic effects. These results suggest that this novel therapeutic tool is applicable to treatment of HER2-overexpressing cancer.
UR - http://www.scopus.com/inward/record.url?scp=3442878768&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3442878768&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2004.03.038
DO - 10.1016/j.canlet.2004.03.038
M3 - Article
C2 - 15279897
AN - SCOPUS:3442878768
SN - 0304-3835
VL - 212
SP - 167
EP - 175
JO - Cancer Letters
JF - Cancer Letters
IS - 2
ER -
