Maitotoxin-photoactive probe binds to membrane proteins in blood cells

Keiichi Konoki, Masaki Hashimoto, Kaori Honda, Kazuo Tachibana, Rie Tamate, Futoshi Hasegawa, Tohru Oishi, Michio Murata

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8 Citations (Scopus)

Abstract

The photoactive and biotinylating ligand was prepared from MTX and maleimide-conjugated Hatanaka reagent with use of Diels-Alder reaction. Blood cells were subjected to affinity labelling experiments using the ligand thus obtained. The labelled band on SDS-PAGE was replaced not by MTX but by brevetoxin B (PbTx2), which suggested the presence of binding proteins in blood 2+ cells. Screening of polyether compounds for MTX inhibitory activity using Ca flux assays in C6 cells disclosed that a synthetic fragment of the hydrophilic portion of MTX inhibited the MTX activity.

Original languageEnglish
Pages (from-to)1007-1017
Number of pages11
JournalHeterocycles
Volume79
Issue numberC
DOIs
Publication statusPublished - Jul 29 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmacology
  • Organic Chemistry

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  • Cite this

    Konoki, K., Hashimoto, M., Honda, K., Tachibana, K., Tamate, R., Hasegawa, F., Oishi, T., & Murata, M. (2009). Maitotoxin-photoactive probe binds to membrane proteins in blood cells. Heterocycles, 79(C), 1007-1017. https://doi.org/10.3987/COM-08-S(D)78