Malignant peripheral nerve sheath tumours: High Ki67 labelling index is the significant prognostic indicator

T. Watanabe, Y. Oda, S. Tamiya, N. Kinukawa, K. Masuda, M. Tsuneyoshi

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Abstract

Aims: We investigated p53, Ki67, MDM2, and p21WAF1/CIP1 in order to evaluate its relationship with prognosis in malignant peripheral nerve sheath tumours (MPNST). Methods and results: In 49 cases of MPNSTs, the immunohistochemical studies of Ki67, p53, MDM2, p21WAF1/CIP1 and polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) with direct sequencing of p53 were performed with the formalin-fixed paraffin-embedded tissue. In 43 cases with survival data available, an evaluation of the prognostic significance of clinicopathological factors was also carried out. A high Ki67 labelling index (LI) (>25%) was correlated with a reduced survival rate in the 43 cases of MPNST (P 0.0106, log-rank test). Furthermore, there was a significant correlation between the Ki67 LI and the immunohistochemical expression of p53 or MDM2. In 17 MPNST cases, PCR amplification of exons 5 through 8 of the p53 gene was successful. One case showed a base change of codon 240 (AGT→AGC), but translated amino acid (Ser) remained unchanged. Multivariate Cox analysis of our series showed that the association of von Recklinghausen's disease, tumour depth, and the presence of rhabdomyoblasts (malignant triton tumour) each had an independent negative impact on overall survival. Conclusion: High Ki67 LI (>25%) was of significant prognostic value in MPNST.

Original languageEnglish
Pages (from-to)187-197
Number of pages11
JournalHistopathology
Volume39
Issue number2
DOIs
Publication statusPublished - Dec 1 2001

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Neurilemmoma
Polymerase Chain Reaction
Neurofibromatosis 1
p53 Genes
Codon
Paraffin
Formaldehyde
Exons
Neoplasms
Multivariate Analysis
Amino Acids

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

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Malignant peripheral nerve sheath tumours : High Ki67 labelling index is the significant prognostic indicator. / Watanabe, T.; Oda, Y.; Tamiya, S.; Kinukawa, N.; Masuda, K.; Tsuneyoshi, M.

In: Histopathology, Vol. 39, No. 2, 01.12.2001, p. 187-197.

Research output: Contribution to journalArticle

Watanabe, T. ; Oda, Y. ; Tamiya, S. ; Kinukawa, N. ; Masuda, K. ; Tsuneyoshi, M. / Malignant peripheral nerve sheath tumours : High Ki67 labelling index is the significant prognostic indicator. In: Histopathology. 2001 ; Vol. 39, No. 2. pp. 187-197.
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