MALS is a binding partner of IRSp53 at cell-cell contacts

Kei Hori, Daijiro Konno, Hisato Maruoka, Kenji Sobue

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26 Citations (Scopus)

Abstract

Insulin receptor substrate p53 (IRSp53) is a key player in cytoskeletal dynamics, interacting with the actin modulators WAVE2 and Mena. Here, we identified a PDZ protein, MALS, as an IRSp53-interacting protein using a yeast two-hybrid screen. A pull-down assay showed that IRSp53 and MALS interact through the PDZ domain of MALS and the C-terminal PDZ-binding sequence of IRSp53. Their interaction in MDCK cells was also demonstrated by co-immunoprecipitation. Immunocytochemistry showed the colocalization of IRSp53 and MALS at cell-cell contacts. Cytochalasin D induced the redistribution of both proteins to the cytosol. Thus, MALS is a partner of IRSp53 anchoring the actin-based membrane cytoskeleton at cell-cell contacts.

Original languageEnglish
Pages (from-to)30-34
Number of pages5
JournalFEBS Letters
Volume554
Issue number1-2
DOIs
Publication statusPublished - Nov 6 2003
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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