Mammalian Elongin A is not essential for cell viability but is required for proper cell cycle progression with limited alteration of gene expression

Katsuhisa Yamazaki, Teijiro Aso, Yoshinori Ohnishi, Ohno Mizuki, Kenji Tamura, Taro Shuin, Shigetaka Kitajima, Yusaku Nakabeppu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Elongin A is a transcription elongation factor that increases the overall rate of mRNA chain elongation by RNA polymerase II. To investigate the function of Elongin A in vivo, the two alleles of the Elongin A gene have been disrupted by homologous recombination in murine embryonic stem (ES) cells. The Elongin A-deficient ES cells are viable, but show a slow growth phenotype because they undergo a delayed mitosis. The cDNA microarray and RNase protection assay using the wildo type and Elongin A-deficient ES cells indicate that the expression of only a small subset of genes is affected in the mutant cells. Taken together, our results suggest that Elongin A regulates transcription of a subset but not all of genes and reveal a linkage between Elongin A function and cell cycle progression.

Original languageEnglish
Pages (from-to)13585-13589
Number of pages5
JournalJournal of Biological Chemistry
Volume278
Issue number15
DOIs
Publication statusPublished - Apr 11 2003

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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