Mammalian Mcm2/4/6/7 complex forms a toroidal structure

Norikazu Yabuta, Naoko Kajimura, Kouta Mayanagi, Michio Sato, Takahito Gotow, Yasuo Uchiyama, Yukio Ishimi, Hiroshi Nojima

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: The Mcm proteins are a family of six homologous proteins (Mcm2-7) that play an important role in DNA replication. They form Mcm4/6/7 and Mcm2/4/6/7 complexes, but their structures are not known. Results: We found that the human Mcm2/4/6/7 tetramer forms a toroidal structure, with a central cavity about 3-4 nm in diameter. Observations were made using electron microscopy, employing the image analysis of single particles. The most predominant averaged image displayed a toroid harbouring four bulges forming corners, one of which was larger than the others. This structure was very similar to the mouse Mcm2/4/6/7 tetramer that was independently prepared and analysed by electron microscopy. These toroidal structures are distinct from that of the Mcm4/6/7 hexamer, which was also examined by electron microscopy. GST(glutathione S-transferase)-pull down and two hybrid experiments suggest that a putative Mcm6-Mcm6 hinge contributes to the formation of the Mcm7/4/6/6/4/7 heterohexamer. Conclusions: The Mcm2/4/6/7 tetramer forms a toroidal structure that is distinct from that of the Mcm4/6/7 hexamer in size and shape.

Original languageEnglish
Pages (from-to)413-421
Number of pages9
JournalGenes to Cells
Volume8
Issue number5
DOIs
Publication statusPublished - May 1 2003
Externally publishedYes

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Electron Microscopy
Glutathione Transferase
DNA Replication
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Yabuta, N., Kajimura, N., Mayanagi, K., Sato, M., Gotow, T., Uchiyama, Y., ... Nojima, H. (2003). Mammalian Mcm2/4/6/7 complex forms a toroidal structure. Genes to Cells, 8(5), 413-421. https://doi.org/10.1046/j.1365-2443.2003.00645.x

Mammalian Mcm2/4/6/7 complex forms a toroidal structure. / Yabuta, Norikazu; Kajimura, Naoko; Mayanagi, Kouta; Sato, Michio; Gotow, Takahito; Uchiyama, Yasuo; Ishimi, Yukio; Nojima, Hiroshi.

In: Genes to Cells, Vol. 8, No. 5, 01.05.2003, p. 413-421.

Research output: Contribution to journalArticle

Yabuta, N, Kajimura, N, Mayanagi, K, Sato, M, Gotow, T, Uchiyama, Y, Ishimi, Y & Nojima, H 2003, 'Mammalian Mcm2/4/6/7 complex forms a toroidal structure', Genes to Cells, vol. 8, no. 5, pp. 413-421. https://doi.org/10.1046/j.1365-2443.2003.00645.x
Yabuta N, Kajimura N, Mayanagi K, Sato M, Gotow T, Uchiyama Y et al. Mammalian Mcm2/4/6/7 complex forms a toroidal structure. Genes to Cells. 2003 May 1;8(5):413-421. https://doi.org/10.1046/j.1365-2443.2003.00645.x
Yabuta, Norikazu ; Kajimura, Naoko ; Mayanagi, Kouta ; Sato, Michio ; Gotow, Takahito ; Uchiyama, Yasuo ; Ishimi, Yukio ; Nojima, Hiroshi. / Mammalian Mcm2/4/6/7 complex forms a toroidal structure. In: Genes to Cells. 2003 ; Vol. 8, No. 5. pp. 413-421.
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