Mammalian Mitochondrial DNA Replicates Bidirectionally from an Initiation Zone

Mark Bowmaker, Ming Yao Yang, Takehiro Yasukawa, Aurelio Reyes, Howard T. Jacobs, Joel A. Huberman, Ian J. Holt

Research output: Contribution to journalArticlepeer-review

156 Citations (Scopus)


Previous data from our laboratory suggested that replication of mammalian mitochondrial DNA initiates exclusively at or near to the formerly designated origin of heavy strand replication, OH, and proceeds unidirectionally from that locus. New results obtained using two-dimensional agarose gel electrophoresis of replication intermediates demonstrate that replication of mitochondrial DNA initiates from multiple origins across a broad zone. After fork arrest near OH, replication is restricted to one direction only. The initiation zone of bidirectional replication includes the genes for cytochrome b and NADH dehydrogenase subunits 5 and 6.

Original languageEnglish
Pages (from-to)50961-50969
Number of pages9
JournalJournal of Biological Chemistry
Issue number51
Publication statusPublished - Dec 19 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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