Mammalian Mitochondrial DNA Replicates Bidirectionally from an Initiation Zone

Mark Bowmaker; Ming Yao Yang; Takehiro Yasukawa; Aurelio Reyes; Howard T. Jacobs; Joel A. Huberman; Ian J. Holt

doi:10.1074/jbc.M308028200

Mammalian Mitochondrial DNA Replicates Bidirectionally from an Initiation Zone

Mark Bowmaker, Ming Yao Yang, Takehiro Yasukawa, Aurelio Reyes, Howard T. Jacobs, Joel A. Huberman, Ian J. Holt

Research output: Contribution to journal › Article › peer-review

156 Citations (Scopus)

Abstract

Previous data from our laboratory suggested that replication of mammalian mitochondrial DNA initiates exclusively at or near to the formerly designated origin of heavy strand replication, O_H, and proceeds unidirectionally from that locus. New results obtained using two-dimensional agarose gel electrophoresis of replication intermediates demonstrate that replication of mitochondrial DNA initiates from multiple origins across a broad zone. After fork arrest near O_H, replication is restricted to one direction only. The initiation zone of bidirectional replication includes the genes for cytochrome b and NADH dehydrogenase subunits 5 and 6.

Original language	English
Pages (from-to)	50961-50969
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	278
Issue number	51
DOIs	https://doi.org/10.1074/jbc.M308028200
Publication status	Published - Dec 19 2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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title = "Mammalian Mitochondrial DNA Replicates Bidirectionally from an Initiation Zone",

abstract = "Previous data from our laboratory suggested that replication of mammalian mitochondrial DNA initiates exclusively at or near to the formerly designated origin of heavy strand replication, OH, and proceeds unidirectionally from that locus. New results obtained using two-dimensional agarose gel electrophoresis of replication intermediates demonstrate that replication of mitochondrial DNA initiates from multiple origins across a broad zone. After fork arrest near OH, replication is restricted to one direction only. The initiation zone of bidirectional replication includes the genes for cytochrome b and NADH dehydrogenase subunits 5 and 6.",

author = "Mark Bowmaker and Yang, {Ming Yao} and Takehiro Yasukawa and Aurelio Reyes and Jacobs, {Howard T.} and Huberman, {Joel A.} and Holt, {Ian J.}",

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language = "English",

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AU - Bowmaker, Mark

AU - Yang, Ming Yao

AU - Yasukawa, Takehiro

AU - Reyes, Aurelio

AU - Jacobs, Howard T.

AU - Huberman, Joel A.

AU - Holt, Ian J.

PY - 2003/12/19

Y1 - 2003/12/19

N2 - Previous data from our laboratory suggested that replication of mammalian mitochondrial DNA initiates exclusively at or near to the formerly designated origin of heavy strand replication, OH, and proceeds unidirectionally from that locus. New results obtained using two-dimensional agarose gel electrophoresis of replication intermediates demonstrate that replication of mitochondrial DNA initiates from multiple origins across a broad zone. After fork arrest near OH, replication is restricted to one direction only. The initiation zone of bidirectional replication includes the genes for cytochrome b and NADH dehydrogenase subunits 5 and 6.

AB - Previous data from our laboratory suggested that replication of mammalian mitochondrial DNA initiates exclusively at or near to the formerly designated origin of heavy strand replication, OH, and proceeds unidirectionally from that locus. New results obtained using two-dimensional agarose gel electrophoresis of replication intermediates demonstrate that replication of mitochondrial DNA initiates from multiple origins across a broad zone. After fork arrest near OH, replication is restricted to one direction only. The initiation zone of bidirectional replication includes the genes for cytochrome b and NADH dehydrogenase subunits 5 and 6.

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 51

ER -

Mammalian Mitochondrial DNA Replicates Bidirectionally from an Initiation Zone

Abstract

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