Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid

Jaakko L.O. Pohjoismäki, J. Bradley Holmes, Stuart R. Wood, Ming Yao Yang, Takehiro Yasukawa, Aurelio Reyes, Laura J. Bailey, Tricia J. Cluett, Steffi Goffart, Smaranda Willcox, Rachel E. Rigby, Andrew P. Jackson, Johannes N. Spelbrink, Jack D. Griffith, Robert J. Crouch, Howard T. Jacobs, Ian J. Holt

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Abstract

We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorporation throughout the lagging strand.

Original languageEnglish
Pages (from-to)1144-1155
Number of pages12
JournalJournal of Molecular Biology
Volume397
Issue number5
DOIs
Publication statusPublished - Apr 2010

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All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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